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30 Cards in this Set
- Front
- Back
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Which tests use SIM's media?
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Indole test, H2S production, Motility test
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Describe how to perform an Indole test
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Inoculate sim's media, incubate, then add Kovac's reagent
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What is the distinctive media in an Indole test?
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trytophan
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What does a positive reaction of an Indole test look like?
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Red color at top
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What is the distinctive media in the H2S test?
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iron, sulfur
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What is a result for H2S test?
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Black precipate
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What does a positive result for a motility test look like?
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Growth away from stab, or turbidity
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What does a positive result for a motility test look like?
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Growth away from stab, or turbidity
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How do you perform a Catalase test?
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streak inoculate a TSA plate, intubate, add hydrogen peroxide
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What is a positive result of the Catalase test?
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If colonies bubble
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What do the Sugar Fermentations test?
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Ability to ferment sugar
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What is distinctive in the media for Sugar Fermentation test?
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Sugar, phenol red, durham tube
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What does a positive Sugar Fermentation test look like?
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Turned to yellow, and/or gas in durham tube
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How do you perform the Triple Sugar Iron Agar test?
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Stab slant and streak top
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What does the Triple Sugar Iron Agar test?
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Ability to ferment glucose and lactose/sucrose
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What are the distinctive media in Triple Sugar Iron Agar?
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phenol red, sugar, agar, ferrous sulfate
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What do the results look like for Triple Sugar Iron Agar?
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butt yellow/slant red glucose only KA
everything yellow, glucose + AA no change nothing K/NC black precipate sulfur H2S cracks produced CO2 gas |
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How do you perform a Citrate test?
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Inoculate green slant and ferment
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What does Citrate test?
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if bacteria uses citrate as sole carbon source and nitrogen as sole source
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What are the distinctive media in a Citrate test?
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simmon's citrate agar, pH indicator
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What does a positive Citrate test look like?
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Media turns blue
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What does the Phenylalanine Deaminace Test (PDT)?
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the ability of an organism to produce the enzyme deaminase
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What are the distinctive media in a PDT test?
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phenylpyruvic acid, slant tubes
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What is the differential indicator in a PDT test?
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ferric chloride
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What does a positive PDT test look like
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deamination of phenylalanine produces a forest green color
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What does a Blood Agar Test (BAP) ?
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It is useful for cultivating fastidious organisms and for determining the hemolytic capabilities of an organism
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What are the distinctive media in Blood Agar?
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general nutrients and 5% sheep blood
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What is the result of alpha hemolysis?
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Alpha-hemolysin partially breaks down the red blood cells and leaves a greenish color behind.
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What is the result of a beta homolysis?
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Beta-hemolysin breaks down the red blood cells and hemoglobin completely. This leaves a clear zone around the bacterial growth
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What happens in a gamma hemolysis?
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If the organism does not produce hemolysins and does not break down the blood cells, no clearing will occur
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