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22 Cards in this Set
- Front
- Back
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what criteria do all media must meet?
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.moisture (maintain osmotic pressure)
2. pH (bacteria: usually 6.5 to 7.5) 3.nutrients 4.be sterile |
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what is the purpose of bile salt in the media?
ex.sodium desoxycholate |
inhibit Gram positive organisms
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what does phenylethylalcohol (PEA) do in the media?
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inhibits Gram negatives
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what is a reducing agent?
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provide decreased O2 tension for anaerobic organisms
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what are the anaerobic indicators?
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1.resazurin
pink = oxidized colorless = reduced 2.methylene blue blue = oxidized colorless = reduced |
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enriched media include____?
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BAP, Chocolate, CYE
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what is chocolate agar (CHOC) ?
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1.enriched media for the isolation of fastidious organisms, especially Haemophilus and pathogenic Neisseria species
2.contains added hemoglobin or isovitalex (NAD) |
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what is the purpose of Thioglycollate broth (THIO)?
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1.supports the growth of both aerobic and anaerobic organisms
2. enhances the growth of organisms found in low numbers in original specimens |
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Colistin Naladixic Acid Agar (CNA)?
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1.selects for Gram positive organisms
2.colistin and nalidixic acid: antibiotics which inhibit gram negatives |
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Phenylethylalcohol Agar (PEA)?
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1.selects for Gram positives
2.phenylethylalcohol: inhibits Gram negatives |
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Modified Thayer Martin Agar (MTM or VCNT)?
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selects for isolation of Neisseria gonorrhoeae (GC) and Neisseria meningitidis
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what are the antibiotics in MTM?
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V= vancomycin inhibits gram positive
C= colistin inhibits gram negatives N= nystatin inhibits yeasts T= trimethoprim inhibits Proteus sp. |
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what is the difference between Modified Thayer Martin Agar (MTM) and Martin Lewis (ML )?
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anisomycin replaces nystatin
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New york city Agar?
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selective for the isolation of GC, also supports the growth of Mycoplasma/Ureaplasma
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MacConkey Agar (MAC)?
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1.selects for Gram negatives
differentiates between lactose fermenters (LF) and non-lactose fermenters (NLF) LF = dark pink colonies NLF = clear colonies |
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Modified Thayer Martin Agar (MTM or VCNT)?
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selects for isolation of Neisseria gonorrhoeae (GC) and Neisseria meningitidis
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what are the antibiotics in MTM?
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V= vancomycin inhibits gram positive
C= colistin inhibits gram negatives N= nystatin inhibits yeasts T= trimethoprim inhibits Proteus sp. |
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what is the difference between Modified Thayer Martin Agar (MTM) and Martin Lewis (ML )?
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anisomycin replaces nystatin
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New york city Agar?
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selective for the isolation of GC, also supports the growth of Mycoplasma/Ureaplasma
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MacConkey Agar (MAC)?
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1.selects for Gram negatives
differentiates between lactose fermenters (LF) and non-lactose fermenters (NLF) LF = dark pink colonies NLF = clear colonies |
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Levine’s Eosin-Methylene Blue Agar (EMB)?
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1. Selects for Gram negatives
2.LF = blue/purple colonies NLF = clear colonies |
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Strep selective agar (SSA)?
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1. Used to select for Group A strep in throat specimens
2. Inhibits NF and allows for the detection of Strep (colistin & SXT) 3. Supplemented with blood to allow in determining hemolytic reactions |
