Mcp Lecture 1

Front 1

Amphipathic lipid

Back 1

Have hydrophobic and hydrophilic parts
polar head groups are hydrophilic
hydrocarbon chain "tails" are hydrophobic

Front 2

polar head group

Back 2

alcohol+phosphate

Front 3

transverse vs lateral diffusion

Back 3

transverse- flip flop, occurs with help of flippases, very rare
lateral diffusion- pairwise exchange of neighboring phospholipid molecules in the same bilayer leaflet

Front 4

melting (transition) temperature

Back 4

temperature where transition between rigid and liquid states occur

Front 5

membrane permeability of lipid bilayer

Back 5

-highly impermeable to polar substances
-impermeable to ions

Front 6

integral vs. peripheral membrane proteins

Back 6

integral is actual inside of the membrane
-transmembrane, mono-layer associated, lipid-linked
peripheral is when the protein is on the outside of the membrane
-protein-attached

Front 7

fluid mosaic model

Back 7

1) lipid bilayer acts as a solvent for membrane proteins
2)membrane proteins are free to diffuse laterally, but cannot flip-flop

Front 8

sources of membrane protein immobility

Back 8

- anchoring the protein to an intracellular structure such as cytoskeleton
- protein-protein interaction between two cells such as neurological of immunological synapses
- integral membrane proteins can be "corraled" to a single area on the cell, such as the apical or basolateral face, by cell-cell interactions such as tight junctions
-anchoring protein to extracellular structure, such as the extracellular matrix

Front 9

oriented monolayer

Back 9

formed by an ampithatic lipid at an air water interface

Front 10

FRAP- florescent recovery after photobleaching

Back 10

attach flourescence to the membrane proteins, zap a small area of membrane to bleach it and then see how long it takes for the proteins to migrate into the bleached area so it flouresces again

Front 11

factors governing membrane fluidity

Back 11

temperature-high temp increases fluidity
length and degree of unsaturation of fatty acid chains-saturated decreases fluidity
cholesterol-decrease

Front 12

detergent solubilization

Back 12

membrane proteins can be removed from bio membranes by the addition of detergents

Front 13

lipid rafts

Back 13

regions of the membrane that are less fluid, and more resistant to solubilization by detergents. In addition to lipids such as sphingolipids and cholesterol, transmembrane domains also show a preference to rafts.
they are implicated in:
-signal transduction
-protein sorting
-recognition
-viral entry or exit from the cell

Front 14

Back 14

sphingomyelin
HYDROPHOBIC PART
fatty acid chain plus phosphoryl choline
HYDROPHILIC PART
hydrocarbon chain of

Front 15

Back 15

Phospholipid
Hydrophobic part- fatty acid chains
Hydrophilic part- phosphorylated alcohol

Front 16

Back 16

Glycolipid
hydrophobic part-fatty acid chain plus hydrocarbon chain of sphingosine
hydrophilic part- one or more sugars

Front 17

Back 17

Cholesterol
Hydrophobic- everything except OH
Hydrophilic- OH