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23 Cards in this Set
- Front
- Back
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Where are group II self-splicing introns only found? On what genes?
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Mitochondria and chloroplasts of rRNA and tRNA genes.
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What is the function of SR proteins?
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To define Exon for splicing of introns. They bind to exonic splicing enhancers.
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What is the purpose of exonic splicing enhancers?
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They promote exon end joining during splicing
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Describe the motifs/domains of SR proteins.
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RRM- RNA Recognizing Motifs & Protein:protein interaction domains.
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What comprises the Cross-Exon recognition Complex?
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U2 and U1 snRNPs, U2AF, and SR proteins.
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True of False: Spliceosomes and Cross-Exon Recognition complex have overlapping elements.
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True: They share U2 and U2AF
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Describe the the function of the U2AF 65kb and 35kb subunits.
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The 65kb unit interacts near the BRANCH POINT. The 35kb interacts AG 3' splice site of the intron.
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What are the protein structures of the RRM of the SR proteins?
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2 alpha helics and 4 beta sheets.
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What is the difference between simple and complex transcription units?
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Simple transcription units have one pattern of RNA splicing, giving rise to only one final mRNA
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Which is more common in humans, complex or simple transcription units?
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Complex (60% roughly)
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What is the major form of splicing that occurs in complex transcription units?
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alternative splicing of exons.
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Describe how sexual dimorphism is affected by sxl gene in drosophila.
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Refer to Text book and notes to confirm.
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True or False: sxl genes don't exist in male flies.
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False.....
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How does the repressor sxl protein affect splicing?
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It affects splicing by inhibiting the binding of U2AF and U2 snRNP, which are crucial for splicing.
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True or false: dsx proteins activate expression of genes required for specific genders.
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FALSE! They are repressors which inhibit the expression of genes related to the undesired gender.
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Does tra contribute to alternative splicing or early poly-adenlyation in the dsx gene?
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Early poly-adenylation.
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What would the addition of multiple sequences of AUUUA in the 3' UTR of an mRNA do?
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Drastically reduce the half-life of an mRNA.
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True or false: some 3' pre-mRNA must be removed before poly(A) tail addition.
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True.
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What does PAP (poly(A) polymerase) need to recognize in order to bind to pre-mRNA?
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A scaffold derived from cleavage and polyadenylation factors associating.
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True or false PAPB I catalyzes rapid addition of A residues by PAP.
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False: it is PAPBII. (PAPB I is cytoplasmic, where mRNA processing doesn't occur.
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U1A's ability to regulate the polyadenlyation of it's own mRNA transcript is an example of what?
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Autoregulatory feedback response.
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True or False: U1A allows for both cleavage and polyadenylation.
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False: It only allows for cleavage, but mRNA is degraded since poly(A) tail can't be added.
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Does polyadenylation regulation or RNA stability regulation occur in TfR?
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RNA stability.
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