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82 Cards in this Set

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substrate (urea)
urea(s)
enzyme (urea)
urease
product (urea)
ammonia NH2
pos (urea)
red/fushia alkaline
neg (urea)
yellow/peach acidic
ph indicator (urea)
phenol red
product (citrate)
ammonia NH2
pos (citrate)
blue alkaline
neg (citrate)
green neutral
ph indicator (citrate)
bromothymol blue
note (citrate)
citrate is only carbon source. ammonia phosphate=NH2PO4.
indirect test.
no such thing as citrase.
substrate (gelatin)
gelatin
enzyme (gelatin)
gelatinase
product (gelatin)
amino acid
pos (gelatin)
liquid
neg (gelatin)
solid
note (gelatin)
is liquid at room temp so refrigerate tube for about 10 minutes and slowly tilt for results.
pos (motility)
fuzzy line
neg (motility)
straight line
note (motility)
use needle and go 2/3 deep in sim's tube so bacteria won't grow around it giving a false reading.
substrate (indole)
trytophane
enzyme (indole)
tryptophanase
pos (indole)
red ring at top of tube
neg (indole)
yellow ring at top of tube
reagent (indole)
kovac's
note (indole)
done in sim's tube
substrate (starch)
starch
enzyme (starch)
amylase
product (starch)
sugars
pos (starch)
yellow with halo in center
neg (starch)
purple
reagent (starch)
iodine
note (starch)
amylase is an exoenzyme
subsrate (MR)
glucose
enzyme (MR)
all glycolysis enzymes
product (MR)
mixed acids
pos (MR)
red
neg (MR)
yellow
ph indicator (MR)
methyl red
reagent (MR)
methyl red
note (MR)
methyl red test.
part of mr-vp test.
ph <4.5.
done in porcelin spot tray.
substrate (VP)
glucose
enzyme (VP)
fermentation enzymes
product (VP)
acetylmethylcarbinol
pos (VP)
red/dark brown like cola
neg (VP)
yellow/gold-no change
reagent (VP)
koh
alphanapthol
note (VP)
vogues proskaver test.
part of mr-vp test.
substrate (carb-dextrose)
dextrose
enzyme (carb-dextrose)
glycolysis/glycolysis & kreb's
product (carb-dextrose)
acid
acid gas
pos (carb-dextrose)
yellow
neg (carb-dextrose)
red/pink
ph indicator (carb-dextrose)
phenol red
note (carb-dextrose)
use durham tube
substrate (carb-lactose)
lactose
enzyme (carb-lactose)
lactase
product (carb-lactose)
glucose
pos (carb-lactose)
yellow
neg (carb-lactose)
red/pink
ph indicator (carb-lactose)
phenol red
substrate (carb-sucrose)
sucrose
enzyme (carb-sucrose)
sucrase
product (carb-sucrose)
glucose
pos (carb-sucrose)
yellow
neg (carb-sucrose)
red/pink
ph indicator (carb-sucrose)
phenol red
substrate (triple sugar iron (tsi)
dextrose)
dextrose
enzyme (triple sugar iron (tsi)
dextrose)
glycolysis
glycolysis & kreb's
product (triple sugar iron (tsi)
dextrose)
acid
acid gas
pos (triple sugar iron (tsi)
dextrose)
alkaline/acid
alkaline/acid gas
neg (triple sugar iron (tsi)
dextrose)
alkaline/alkaline
note (triple sugar iron (tsi)
dextrose)
stab the butt and streak the slant
results recorded as slant/butt.
substrate (triple sugar iron (tsi)
lactose/sucrose)
lactose/sucrose
enzyme (triple sugar iron (tsi)
lactose/sucrose)
glycolysis/glycolysis & kreb's
product (triple sugar iron (tsi)
lactose/sucrose)
yellow
acid
acid gas
pos (triple sugar iron (tsi)
lactose/sucrose)
yellow
acid/acid
acid/acid gas
neg (triple sugar iron (tsi)
lactose/sucrose)
fushia
alkaline/alkaline
substrate (triple sugar iron (tsi)
FeSO4)
FeSO4
enzyme (triple sugar iron (tsi)
FeSO4)
respiratory chain
product (triple sugar iron (tsi)
FeSO4)
hydrogen sulfide gas H2S
note (triple sugar iron (tsi)
FeSO4)
NEVER alkaline/alkaline
fushia