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82 Cards in this Set
- Front
- Back
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substrate (urea)
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urea(s)
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enzyme (urea)
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urease
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product (urea)
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ammonia NH2
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pos (urea)
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red/fushia alkaline
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neg (urea)
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yellow/peach acidic
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ph indicator (urea)
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phenol red
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product (citrate)
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ammonia NH2
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pos (citrate)
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blue alkaline
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neg (citrate)
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green neutral
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ph indicator (citrate)
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bromothymol blue
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note (citrate)
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citrate is only carbon source. ammonia phosphate=NH2PO4.
indirect test. no such thing as citrase. |
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substrate (gelatin)
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gelatin
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enzyme (gelatin)
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gelatinase
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product (gelatin)
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amino acid
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pos (gelatin)
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liquid
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neg (gelatin)
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solid
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note (gelatin)
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is liquid at room temp so refrigerate tube for about 10 minutes and slowly tilt for results.
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pos (motility)
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fuzzy line
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neg (motility)
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straight line
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note (motility)
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use needle and go 2/3 deep in sim's tube so bacteria won't grow around it giving a false reading.
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substrate (indole)
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trytophane
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enzyme (indole)
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tryptophanase
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pos (indole)
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red ring at top of tube
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neg (indole)
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yellow ring at top of tube
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reagent (indole)
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kovac's
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note (indole)
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done in sim's tube
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substrate (starch)
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starch
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enzyme (starch)
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amylase
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product (starch)
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sugars
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pos (starch)
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yellow with halo in center
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neg (starch)
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purple
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reagent (starch)
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iodine
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note (starch)
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amylase is an exoenzyme
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subsrate (MR)
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glucose
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enzyme (MR)
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all glycolysis enzymes
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product (MR)
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mixed acids
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pos (MR)
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red
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neg (MR)
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yellow
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ph indicator (MR)
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methyl red
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reagent (MR)
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methyl red
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note (MR)
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methyl red test.
part of mr-vp test. ph <4.5. done in porcelin spot tray. |
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substrate (VP)
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glucose
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enzyme (VP)
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fermentation enzymes
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product (VP)
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acetylmethylcarbinol
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pos (VP)
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red/dark brown like cola
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neg (VP)
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yellow/gold-no change
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reagent (VP)
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koh
alphanapthol |
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note (VP)
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vogues proskaver test.
part of mr-vp test. |
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substrate (carb-dextrose)
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dextrose
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enzyme (carb-dextrose)
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glycolysis/glycolysis & kreb's
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product (carb-dextrose)
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acid
acid gas |
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pos (carb-dextrose)
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yellow
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neg (carb-dextrose)
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red/pink
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ph indicator (carb-dextrose)
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phenol red
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note (carb-dextrose)
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use durham tube
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substrate (carb-lactose)
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lactose
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enzyme (carb-lactose)
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lactase
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product (carb-lactose)
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glucose
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pos (carb-lactose)
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yellow
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neg (carb-lactose)
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red/pink
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ph indicator (carb-lactose)
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phenol red
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substrate (carb-sucrose)
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sucrose
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enzyme (carb-sucrose)
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sucrase
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product (carb-sucrose)
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glucose
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pos (carb-sucrose)
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yellow
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neg (carb-sucrose)
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red/pink
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ph indicator (carb-sucrose)
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phenol red
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substrate (triple sugar iron (tsi)
dextrose) |
dextrose
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enzyme (triple sugar iron (tsi)
dextrose) |
glycolysis
glycolysis & kreb's |
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product (triple sugar iron (tsi)
dextrose) |
acid
acid gas |
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pos (triple sugar iron (tsi)
dextrose) |
alkaline/acid
alkaline/acid gas |
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neg (triple sugar iron (tsi)
dextrose) |
alkaline/alkaline
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note (triple sugar iron (tsi)
dextrose) |
stab the butt and streak the slant
results recorded as slant/butt. |
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substrate (triple sugar iron (tsi)
lactose/sucrose) |
lactose/sucrose
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enzyme (triple sugar iron (tsi)
lactose/sucrose) |
glycolysis/glycolysis & kreb's
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product (triple sugar iron (tsi)
lactose/sucrose) |
yellow
acid acid gas |
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pos (triple sugar iron (tsi)
lactose/sucrose) |
yellow
acid/acid acid/acid gas |
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neg (triple sugar iron (tsi)
lactose/sucrose) |
fushia
alkaline/alkaline |
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substrate (triple sugar iron (tsi)
FeSO4) |
FeSO4
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enzyme (triple sugar iron (tsi)
FeSO4) |
respiratory chain
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product (triple sugar iron (tsi)
FeSO4) |
hydrogen sulfide gas H2S
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note (triple sugar iron (tsi)
FeSO4) |
NEVER alkaline/alkaline
fushia |
