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27 Cards in this Set
- Front
- Back
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Complex I other name?
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NADH-Q Reductase Complex or NADH Dehydrogenase/NAD+ Reductase
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Complex II other name?
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Succinate Dehydrogenase
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Complex III other name?
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QH2-cytochrome c Reductase complex or Cytochrome reductase
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Complex IV other name?
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Cytochrome oxidase complex
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Complex V other name?
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ATP Synthase
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Components of Complex I
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-NADH
-Coenzyme Q (Ubiquinone) -Fe-S |
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Components of Complex II
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-FADH2
-Coenzyme Q -Succinate -Fe-S Complex II is membrane bound! |
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Components of Complex III
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-Cytochrome b & c
-Fe-S |
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Components of Complex IV
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-Cytochrome a+a3
-Binds O2 -Cu+Fe (Cu++ reduced to Cu+) |
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Components of Complex V
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-FeF
-(ADP + Pi --> ATP) |
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Coenzyme Q carry e- where?
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From To
- Complex I&II --> Complex III |
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Cytochrome c carry e- where?
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Complex III --> Complex IV
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Standard reduction potential(E)?
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More (-)E value -- reductant member will donate e-
More (+)E value --Oxidant member will accept e- |
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P/O # for NADH and FADH2?
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NADH = 3
FADH2 = 2 |
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Describe ATP Synthase?
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-Most abundant Mito protein
-3 ATP's per revolution -Occurs only with H+ Flow -1 ATP per 2 protons across membrane |
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What happens to ATP synthase in the Absence of H+?
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ATP synthase breaks down ATP in the reverse process called - Mitochondria ATPase.
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Inhibition of oxidative phosphorlyation includes what?
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Absence of e- flow-> no H+ flow ->no ATP synthesis.
Absence of ATP synth want for ADP and Pi-> no e- flow. |
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Effect of Rotenone?
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Inhibit e- flow from Complex I (Fe-S complexes) to Ubiquinone
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Effect of Barbiturates?
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Inhibit flow through Complex I
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Effect of Antimycin-A (streptomycete)?
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Blocks e- flow through Complex III.
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Effect of Cyanide, azide? and CO?
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-Bind to Fe+++ in cytochrome a/a3 causing Fe to oscillate btw +++ and ++
- Bind to Fe++ |
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What does an uncoupler do?
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Prevents ATP synthesis without inhibiting e- flow
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Effect of Dinitrophenol and Pentachlorphenol.
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Dissipate proton gradient by transposing H+ across inner membrane w/o ATP synthase.
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Describe Thermogenin.
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-Normally occurring uncoupler
-Brown Adipose Tissue -Exploit High -DeltaG from H+ passing down gradient w/o synthesizing ATP -Temperature control |
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Why is bilirubin bad?
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-To high of conc. result in hyperbilirubinemia.
-Uncoupling occurs w/brain damage in infants |
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How does arsenate effect the process?
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Competes with Pi for Pi transporter.
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Effect of pentachlorophenol?
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-NADH oxidation & O2 consumption are elevated.
- no ATP made -individual is hyperthermic |
