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16 Cards in this Set
- Front
- Back
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What is a common selectable marker?
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B-lactamase which makes the cell resistant to ampicillin
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How many bp's are usually in a restriction site overhang?
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4-8 bp's
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What is a polylinker?
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The site of DNA to be cloned can be isolated via multiple restriction sites.
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What is the equation to determine the probability of finding a recognition site?
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P=(1/4)'n
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In vitro, what serves as a primer for reverse transcriptase?
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polydT
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What are restriction maps?
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A map of the various restriction sites around a particular gene
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What is a RFLP?
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Cutting up the gene with restriction enzymes and gel electrophoresing them to see if there are any polymorphisms.
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What does ethidium bromide do?
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Binds only to double stranded DNA or RNA.
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What is the mutation in sickle cell anemia?
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Glu6Val
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What gene is affected in the SCA?
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the Beta globin gene
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For the Sanger Method of DNA sequencing, what is specifically used?
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dideoxyribonucleoside triphosphate
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What is a Northern blot not good at?
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determing which end is the right end or differentiating between RNA which are different by just a couple of bp's
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What are pseudogenes?
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Open reading frames on genes but are not expressed.
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What is the thermo stable polymerase?
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Thermus aquaticus
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What is the temperature range for PCR?
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94-55
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What kind of DNA is used for microarrays?
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cDNA
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