Mcb 2000 Enzymes For Final Exam

Front 1

Glucokinase

Reaction, Regulation, Pathway, Location, Coenzymes, Active state

Back 1

Glucokinase

Glucose -> Glucose 6P / ATP->ADP, Induced- controlled by insulin, Many, Cytoplasm (Liver), Fed state

Front 2

Hexokinase

Reaction, Regulation, Pathway, Location, Coenzymes, Active state

Back 2

Hexokinase

Glucose -> Glucose 6P / ATP->ADP, FBI by product, Many, Cytoplasm, None, Fed state

Front 3

Phosphofructokinase-1 (lolPFK-1)

Reaction, Regulation, Pathway, Location, Coenzymes, Active state

Back 3

PFK-1

Fructose 6P -> Fructose 1,6BisP / ATP->ADP, Allosteric Regulation - activated by AMP/ADP/Fructose 2,6BisP, inhibited by ATP and citrate, Glycolysis, Cytoplasm, None, Fed state

Front 4

Pyruvate Kinase

Reaction, Regulation, Pathway, Location, Coenzymes, Active state

Back 4

Pyruvate Kinase

PEP + ADP -> Pyruvate + ATP, Allosteric and Hormonal - activated by Fructose 1,6BisP, inhibited by ATP and alanine, dephosphorylated, Glycolysis, None, Fed state

Front 5

Glyceraldehyde-3P Dehydrogenase

Reaction, Regulation, Pathway, Location, Coenzymes, Active state

Back 5

Glyceraldehyde-3P Dehydrogenase

Glyceraldehyde-3P + Pi -> 1,3 Bisphosphoglycerate / NAD->NADH, None, Glycolysis, Cytoplasm, None

Front 6

Lactate Dehydrogenase

Reaction, Regulation, Pathway, Location, Coenzymes, Active state

Back 6

Lactate Dehydrogenase

Pyruvate -> Lactate / NADH -> NAD, NAD/NADH ratio - regenerates NAD+, Anaerobic Respiration, Cytosol, None

Front 7

Phosphofructokinase-2 (lolPFK-2)

Reaction, Regulation, Pathway, Location, Coenzymes, Active state

Back 7

PFK-2

Fructose 6P -> Fructose 2,6BisP / ATP->ADP, Dephosphorylated in Fasting, Phosphorylated in Fed, Glycolysis, None, Depends on Circumstances

Front 8

Pyruvate Dehydrogenase

Reaction, Regulation, Pathway, Location, Coenzymes, Active state

Back 8

Pyruvate Dehydrogenase

Pyruvate -> AcetylCoA / NAD->NADH, Allosteric and Hormonal - activated by CoA and NAD+, inhibited by AcetylCoA ATP and NADH, between Glycolysis and TCA Cycle, in Mitochondria, Thiamine, Lipoate, FAD, Dephosphorylated in Fed State

Front 9

Citrate Synthase

Reaction, Regulation, Pathway, Location, Coenzymes, Active state

Back 9

Citrate Synthase

Oxalacetate + AcetylCoA -> Citrate, Substrate Concentration and FBI by product, TCA Cycle, Mitochonrial Matrix, None,

Front 10

Isocitrate Dehydrogenase

Reaction, Regulation, Pathway, Location, Coenzymes, Active state

Back 10

Isocitrate Dehydrogenase

Isocitrate -> alphaKetoglutarate / NAD->NADH, Allosteric - activated by ADP and NAD, inhibited by ATP and NADH, TCA Cycle, Mitochondrial Matrix, None, Responds to energy levels

Front 11

alphaKetoglutarate Dehydrogenase

Reaction, Regulation, Pathway, Location, Coenzymes, Active state

Back 11

alphaKetoglutarate Dehydrogenase

alphaKetoglutarate -> SuccinylCoA / NAD->NADH, activated by AMP, inhibited by NADH and succinylCoA, TCA Cycle, Mitochondrial Matrix, ThiaminePP, CoASH, Lipoate, FAD, NAD+

Front 12

Succinate Dehydrogenase

Reaction, Regulation, Pathway, Location, Coenzymes, Active state

Back 12

Succinate Dehydrogenase

Succinate->Fumarate / FAD->FADH2, None, TCA Cycle, Inner Mitochondrial Membrane

Front 13

SuccinylCoA Synthetase

Reaction, Regulation, Pathway, Location, Coenzymes, Active state

Back 13

SuccinylCoA Synthetase

SuccinylCoA -> Succinate, None, TCA Cycle, Mitochondrial Matrix

Front 14

Malate Dehydrogenase

Reaction, Regulation, Pathway, Location, Coenzymes, Active state

Back 14

Malate Dehydrogenase

L-Malate -> Oxalacetate / NAD->NADH, None, TCA Cycle, Mitochondrial Matrix

Front 15

Glycogen Synthase

Reaction, Regulation, Pathway, Location, Coenzymes, Active state

Back 15

Glycogen Synthase

UDP-Glucose->Glycogen, Elongates Glycogen after first 6-8 residues, Glycogen synthesis, Active while Dephosphorylated - Fed state

Front 16

Glycogen Phosphorylase

Reaction, Regulation, Pathway, Location, Coenzymes, Active state

Back 16

Glycogen Phosphorylase

Glycogen -> Glucose 1P, Removes residues from Glycogen, Cleaves 1-4 linkages and not the 4 closest to branch point 'cause of steric hindrance, Active while Phosphorylated - Fasting state, Glycogen Degradation

Front 17

Glucose 6-Phosphatase

Reaction, Regulation, Pathway, Location, Coenzymes, Active state

Back 17

Glucose 6-Phosphatase

Glucose 6P -> Glucose, Glycogen Degradation

Front 18

AcetylCoA Carboxylase

Reaction, Regulation, Pathway, Location, Coenzymes, Active state

Back 18

AcetylCoA Carboxylase

AcetylCoA -> MalonylCoA / ATP->ADP, Allosteric and hormonal - activated by citrate and insulin, inhibited by fattyacylCoA and glucagon, Lipid Synthesis, Cytoplasm, Dephosphorylated in Fed state

Front 19

Citrate Lyase

Reaction, Regulation, Pathway, Location, Coenzymes, Active state

Back 19

Citrate Lyase

Citrate -> Acetyl CoA + Oxalacetate / ATP->ADP, Lipid Synthesis, Cytoplasm

Front 20

Fatty Acid Synthase

Reaction, Regulation, Pathway, Location, Coenzymes, Active state

Back 20

Fatty Acid Synthase

MalonylCoA -> Palmitate / NADPH->NADP, extends the fatty acid chain, requires a lot of NADPH, Fatty Acid Synthase complex, Lipid Synthesis, Fed state

Front 21

Malate Dehydrogenase (Cytosolic)

Reaction, Regulation, Pathway, Location, Coenzymes, Active state

Back 21

Malate Dehydrogenase (Cytosolic)

Oxalacetate -> Malate / NADH->NAD, Lipid Synthesis, Cytoplasm

Front 22

Malic Enzyme

Reaction, Regulation, Pathway, Location, Coenzymes, Active state

Back 22

Malic Enzyme

Malate -> Pyruvate / NADP->NADPH, Lipid Synthesis, Cytoplasm, Fed state

Front 23

Glycerol Kinase

Reaction, Regulation, Pathway, Location, Coenzymes, Active state

Back 23

Glycerol Kinase

ATP + Glycerol -> ADP + Glycerol Phosphate, ehhhh?

Front 24

Carnitine:palmitoyltransferase-1 (lolCPT1)

Reaction, Regulation, Pathway, Location, Coenzymes, Active state

Back 24

CPT1

Fatty Acyl CoA -> Fatty Acyl Carnitine, inhibited by Malonyl CoA, Fatty Acid Oxidation

Front 25

Lipoprotein Lipase

Reaction, Regulation, Pathway, Location, Coenzymes, Active state

Back 25

Lipoprotein Lipase

Triglycerol -> Glycerol + 3FAs

Front 26

Glucose 6-Phosphate Dehydrogenase

Reaction, Regulation, Pathway, Location, Coenzymes, Active state

Back 26

Glucose 6P Dehydrogenase

Glucose 6P -> 6phosphonoglucosdfdsalfjdskalactone / NADP->NADPH, Inhibited by NADPH, Pentose Phosphate Pathway

Front 27

betaHydroxy-betaMethyl GlutarylCoA Reductase (HMG-CoA Reductase)

Reaction, Regulation, Pathway, Location, Coenzymes, Active state

Back 27

HMG-CoA Reductase

AcetylCoA -> Mevalonate/ 2NADPH->2NADP, Phosphorylation by AMP activated kinase inactivates, Degradation -half life 3 hours, Gene Expression, Cholesterol Synthesis

Front 28

Pyruvate Carboxylase

Reaction, Regulation, Pathway, Location, Coenzymes, Active state

Back 28

Pyruvate -> Oxalacetate / ATP->ADP, Allosterically activated by AcetylCoA, Gluconeogenesis, Mitochondria, Biotin

Front 29

Phosphoenolpyruvate Carboxykinase (lolPEPCK)

Reaction, Regulation, Pathway, Location, Coenzymes, Active state

Back 29

PEPCK

Oxalacetate -> Phosphoenolpyruvate / GTP->GDP, Gluconeogenesis, Cytoplasm

Front 30

Fructose 1,6BisPhosphatase

Reaction, Regulation, Pathway, Location, Coenzymes, Active state

Back 30

Fructose 1,6BisP Phosphatase

Fructose 1,6BisP -> Fructose 6P, Allosteric - activated by citrate, inhibited by fructose 2,6 bisphosphate and AMP, Gluconeogenesis, Cytoplasm

Front 31

Glucose 6-Phosphatase

Reaction, Regulation, Pathway, Location, Coenzymes, Active state

Back 31

Glucose 6-phosphatase

Glucose 6P -> Glucose, Substrate level regulation, Gluconeogenesis, Cytoplasm

Front 32

Alcohol Dehydrogenase

Reaction, Regulation, Pathway, Location, Coenzymes, Active state

Back 32

Alcohol Dehydrogenase

Ethanol -> Acetaldehyde / NAD->NADH, Ethanol Metabolism

Front 33

Aldehyde Dehydrogenase

Reaction, Regulation, Pathway, Location, Coenzymes, Active state

Back 33

Aldehyde Dehydrogenase

Acetaldehyde -> Acetate / NAD->NADH

Front 34

Cytochrome P450 2E1

Reaction, Regulation, Pathway, Location, Coenzymes, Active state

Back 34

Cytochrome P450 2E1

Ethanol -> Acetaldehyde, for high concentrations of Ethanol