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7 Cards in this Set

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Staphylococcus Family/genus/species
Family: micrococcaceau
Genus: Staphylococcus
Species; aureus, epidermidis, saprophyticus, 31 others.
Common characteristics of Staph
Gram stain = clusters, gram positive; nice and round like grapes.

Catalase Positive

Growth requirements = not fastidious; facultative anaerobe, grows any media
Staph colonial morphology
white, round, creamy, opaque, conves, with continued incubation it enlarges
how does coag/staphylatex/agglutination test differentiate staphylococcus species?
positive = s. aureus
negative = s. epidermidis or saprophyticus
principe of coag test
coagulase is an enzyme that coagulates plasma = produces clumps.

s. aureus produces coagulase; s. epi and saproph don't.

First do slide test for bound; if neg, then do tube test for free coag. if STILL neg, report as COAG NEG STAPH
principle of staphylatex
beads coated with fibrinogen clump if coag is produced (staph aureus +)

also coated with anti-protein A antibody. S. aureus produce protein A so that will clump too.
what does DNAse agar contain?
DNA and methyl-green indicator.
-S. aureus breaks down DNA so it turns CLEAR.

(intact DNA remains green)