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7 Cards in this Set
- Front
- Back
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Staphylococcus Family/genus/species
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Family: micrococcaceau
Genus: Staphylococcus Species; aureus, epidermidis, saprophyticus, 31 others. |
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Common characteristics of Staph
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Gram stain = clusters, gram positive; nice and round like grapes.
Catalase Positive Growth requirements = not fastidious; facultative anaerobe, grows any media |
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Staph colonial morphology
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white, round, creamy, opaque, conves, with continued incubation it enlarges
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how does coag/staphylatex/agglutination test differentiate staphylococcus species?
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positive = s. aureus
negative = s. epidermidis or saprophyticus |
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principe of coag test
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coagulase is an enzyme that coagulates plasma = produces clumps.
s. aureus produces coagulase; s. epi and saproph don't. First do slide test for bound; if neg, then do tube test for free coag. if STILL neg, report as COAG NEG STAPH |
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principle of staphylatex
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beads coated with fibrinogen clump if coag is produced (staph aureus +)
also coated with anti-protein A antibody. S. aureus produce protein A so that will clump too. |
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what does DNAse agar contain?
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DNA and methyl-green indicator.
-S. aureus breaks down DNA so it turns CLEAR. (intact DNA remains green) |
