Use LEFT and RIGHT arrow keys to navigate between flashcards;
Use UP and DOWN arrow keys to flip the card;
H to show hint;
A reads text to speech;
7 Cards in this Set
- Front
- Back
|
Precursor ions |
The ions with intact mass of the analyte |
|
|
Fragment ions |
Ions generated from fragmentation of the precursor ions. |
|
|
Ion suppression |
Suppression of ionisation of low abundance analytes by high abundance analytes in the ion source. Seen in soft ionisation techniques. |
|
|
Metabolomics |
Metabolite extraction Key: rapid enzyme sequencing Chromatography Key: separation GC: volatility HILIC: Polarity RP: Hydrophobicity Mass Spectrometry Key: sensitivity Specificity TOF, Orbitrap: mass resolution Triple quad: fragmentation Data analysis Key: metabolite identification Challenges Metabolite misidentification: fragments mimicking real metabolites Identifying unknowns: Need MS/MS or NMR |
|
|
Masses in mass spectrometry |
Avg mass Exact mass Mono isotopic mass Accurate mass |
|
|
Basic steps in metabolomics |
Quenching: to stop enzyme activity and interconversion between metabolites. Extraction: extraction of metabolites into solvent. Analysis: Mass spectrometry, NMR, Enzyme assays |
|
|
Sangers reagent |
Fluorodinitrobenzene FDNM |
