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8 Cards in this Set
- Front
- Back
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What is the application for the MacConkey Agar?
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Used to isolate and differentiate members of the enterobacteria based on the ability to ferment lactose.
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What is the media?
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Lactose. bile salts. neutral red. and crystal violet.
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What is the purpose of bile salts and crystal violet in the media?
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Inhibit growth of Gram-positive bacteria.
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What is the purpose of neutral red dye in the media?
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A pH indicator that is colorless above pH of 6.8 and red at pH less than 6.8. Lactose fermenters turn a shade of red on the agar.
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What is the interpretation and Presumptive ID for a result of Poor growth or no growth?
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Organism is inhibited by crystal violet and/or bile salt. Gram-postive.
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What is the interpretation and Presumptive ID for a result of good growth?
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Organism is not inhibited by crystal violet or bile. Gram-negative.
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What is the interpretation and Presumptive ID for a result of Pink to red growth with or without bile precipitate?
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Organism produces acid from lactose fermentation. Probable coliform.
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What is the interpretation and Presumptive ID for a result of colorless growth?
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Organism does not ferment lactose. No reaction. Noncoliform.
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