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11 Cards in this Set

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  • Back

Macconkey agar

-selective and differential medium


-lactose, bile salts, neutral red, Crystal violet


-isolate and differentiate enterobacteriaceae

Gram positive bacteria

-bile salts and Crystal violet inhibit growth


Neutral red dye

- is a pH indicator that is colorless above pH of 6.8


- red at pH less than 6.8

Dye turns red

-acid accumulating from lactose fermentation


-nonfermentators retain their color


-probable coliform

Colorless growth

-organism does not ferment lactose


- no reaction


- noncoliform

Good growth (g)

-organism is not inhibited by Crystal violet or bile


-gram neg.

Poor growth or no growth (p)

- organism is inhibited by Crystal violet and or bile


-gram positive

MacConkey Agar and emb agar

Selective for gram negative organisms and both contain indicators to differentiation lactose fermentation and non.

Emb is commonly used

To test for fecal contamination


Presences of coloiform

The top and right lactose fermentation because pink


The bottom ones non


The top and right are gram negative lactose fermentation. Difference in color is due to the amount of acid produced



The bottom and left are gram positive inhibited by eosin y and methylene blue