Reading...
Play button
Play button
Use LEFT and RIGHT arrow keys to navigate between flashcards;
Use UP and DOWN arrow keys to flip the card;
H to show hint;
A reads text to speech;
19 Cards in this Set
- Front
- Back
|
Fresh/preserved stool:
- exam method - look for what? |
Macroscopy
- Blood - Mucus - Worms - Proglottids Microscopy - Cysts - Eggs - Larvae - Trophozoites |
|
|
Sigmoidoscopic material:
- exam method - look for what? |
Scraped or aspirated mucosal material
Motile ENTAMOEBA trophozoites |
|
|
Perianal Specimen:
- exam method - look for what? |
Cellulose tape slide or
Anal swap Pinworms |
|
|
Duodenal aspirates:
- exam method - look for what? |
Endoscope or String test
Strongyloides larvae Worm eggs Giardia |
|
|
How do you check for parasitic infections of blood?
What do you look for? x4 |
Blood films (thick and thin) with Giemsa and/or hematoxylin stains.
Malaria Trypanasomiasis Filariasis Leishmaniasis |
|
|
What tests are useful for detecting intracellular parasites?
What are some of these parasites |
Tissue biopsy w/ Giemsa stain of:
Bone marrow Liver Spleen Leishmaniasis Toxoplasma |
|
|
What test is useful for detecting microfilariae?
Example of organism |
Skin snips
Onchocerca volvulus |
|
|
PCR use for Plasmodium Vivax:
- what do you look for? |
Circumsporozoite gene
|
|
|
PCR use for Leishmania sp:
- what do you look for? |
kDNA minicircle sequence
|
|
|
PCR use for Trypanosoma cruzi:
- what do you look for? |
kDNA minicircle sequence
|
|
|
PCR use for Toxoplasma gondii:
- what do you look for? |
B1 repetitive gene
p30 surface antigen Recombinant DNA sequences |
|
|
PCR use for Entamoeba histolytica:
- what do you look for? |
p145 tandem repeat sequence
|
|
|
Describe the value of doing cultures for parasites.
|
Not commonly used
Too difficult and/or cumbersome to cultivate |
|
|
Describe the value of doing Animal inoculation for parasites.
|
Not practical
|
|
|
Describe the value of doing xenodiagnosis for parasites.
|
Good for Chagas disease using uninfected reduvid bugs.
|
|
|
Describe the steps in making an Enzyme immunoassay for the ELISA test
|
1. Parasite antigen pre-coated onto an ELISA plate
2. Patient serum which contains antibodies mixed in 3. Anti-human immmunoglobulin coupled to an enzyme indicates positive test. |
|
|
Indirect immunofluorescence assay is used to detect _________ in ________________.
|
antibodies
serum or other bodily fluid |
|
|
Describe the steps in making an Enzyme immunoassay for the EIA test.
|
1. Parasite antigens pre-coated onto plastic bead
2. Patient serum which contains antibodies mixed in. 3. Anti-human immunoglobulin coupled to an enzyme in positive test. |
|
|
Immunoblot is used to detect ____________
|
the presence of an antigen
|
