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16 Cards in this Set
- Front
- Back
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What kinds of signals are conveyed to cells by the ECM? x 2
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1. Positional information
(Where am I? Am I moving?) 2. Differentiation information. (Who am I? What am I?) |
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What is the structure of integrins?
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Heterodimeric proteins
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What is the function of integrins?
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Mediate cell/matrix and cell/cell interaction.
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What are the subunits of integrins called?
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alpha and beta
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What is the purpose of the beta subunit integrin?
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Connect the outside of the cell and the cytoskeletal elements of the cell
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Integrin interaction with ECM motifs are dependent on what?
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Divalent cation dependent (Ca2+ and/or Mg2+)
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What is "inside-out" signaling (or affinity modulation)?
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The dynamic change in integrin affinity state by cells.
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What causes the low affinity state of integrins?
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Cytoplasmic domains of the alpha and beta subunit form a "salt bridge"
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What causes the high affinity state of integrin?
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The interaction of cytoplasmic domains with adapter protein defeats the salt bridge.
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Give three examples of adapter proteins that defeat the salt bridge.
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Paxillin
Endonexin Cytohexin |
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What is the target integrin associated with platelet aggregation?
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Alpha 2b Beta 3
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What domain binds fibronectin to integrins?
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RGD (or LAV)
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Describe "outside in" signaling.
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1. Fibronectin binds with integrins on cell surface.
2. Which mediates binding of signal proteins and/or adapter proteins. 3. Focal Adhesion Effector Complex binds the beta subunit and this allows actin to bind. 4. Amplification by recruitment of other integrin/ECM interactions. |
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What are the three parts of the Focal Adhesion Effector complex?
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1. Signaling Proteins
2. Docking/Adaptor proteins 3. Actin Binding Proteins |
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What is syndecan?
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A cell surface proteoglycan
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Name three playas that regulate Integrin "outside-in" signaling.
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1. FAK (Focal Adhesion Kinase)
2. Rho-GTPase 3. Syndecan |
