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30 Cards in this Set

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Community-acquired Pneumonia
What are the symptoms?
fever and chills, chest pain, cough, headache, sweating from high fever
Community-acquired Pneumonia
What are the specimens collected?
sputum, BAL, pleural fluid, tracheal aspirate
Community-acquired Pneumonia
Media/Incubation?
BAPss/CO2
CHOC/CO2
MAC
smear with Q-score
Community-acquired Pneumonia
What are the common pathogens in Neonate?
Chlamydia trachomatis
Community-acquired Pneumonia
What are the common pathogens in young children?
RSV
Influenza
(mostly viruses)
Community-acquired Pneumonia
What are the common pathogens in Teen to young adult?
Chlamydia pneumoniae
Mycoplasma pneumoniae
Influenza virus
Community-acquired Pneumonia
What are the common pathogens in older adult?
S. pneumoniae
H. influenzae
Respiratory viruses
Gram neg rods
Acute bronchitis
What are the common pathogens?
Respiratory viruses
Mycoplasma pneumoniae
Chlamydia pneumoniae
Bordetella pertussis
Haemophilus influenzae
4 mechanisms by which LRT infections occur.
1. Invasion from upper airway
2. Inhalation of infectious particles
3. Aspiration of oral or gastric contents
4. Hematogenous spread
3 host defence mechanisms in the LRT.
1. Coughing
2. Alveolar macrophages
3. Leukocytes, monocytes
4 virulence factors in LRT.
1. Adherence
2. Toxins
3. Capsule
4. Intracellular multiplication
Chronic pneumonia
Common pathogens?
Mycobacterium tuberculosis
Fungi
Non tuberculosis mycobacteria
Hospital-acquired pneumonia
Common pathogens?
Klebsiella sp.
Enterobacteriaceae
S. aureus
S. pneumo
Ps. aeruginosa
Anaerobes
Legionella sp.
Aspiration pneumonia
Common pathogens?
Anaerobes
S. aureus
Pseudomonads
Enterobacteriaceae
Opportunistic pathogens that infect the respiratory tracts of immunocompromised patients
Mycobacterium tuberculosis
Pneumocytis carinii
Chlamydia pneumoniae
Mycoplasma pneumoniae
Legionella pneumophila
Two step procedure before inoculation of sputum.
Decontamination
Liquefaction
What is the purpose of decontamination of sputum?
To suppress normal flora (long incubation)
with KOH
What is the purpose of liquefaction of sputum?
To release the organisms
Pathogens common in cystic fibrosis patients?
Ps. aeruginosa
Burkholderia cepacia
S. aureus
H. influenzae
Aspergillus sp.
viruses
Media used to detect H. influenzae in CF patients.
Horse blood agar with bacitracin
Media used to detect S. aureus in CF patients.
MSA (yellow)
What is added to culture Legionella pneumophila?
L-cysteine and iron salts
What is used to grow Mycobacterium tuberculosis?
Lowenstein-Jensen (egg based)
Middlebrook THIO or THII (serum or agar based)
Broth, blood culture (fluid)
What is used to stain M. tuberculosis?
Ziehl-Neelson
Kinyouns
Auramine-rhodamine
What is used to stain Pneumocystis carinii?
Fungi-fluor
calcofluor white
giemsa
methanamine silver
toluidine blue O
What type of agar is used to grow Mycoplasma pneumoniae?
SP4
H agar
Encapsulated bacteria in LRT.
S. aureus
Ps. aerug
H. influenzae
Adherence bacteria in LRT.
What is used to adhere?
S. pyogenes: M proteins
Listeria: fimbriae
S. aureus, viridans: lipoteichoic acid
What does Ps. aerug use as toxin in LRT?
Exotoxin A
Pyocyanins
What are the symptoms of bronchiolitis?
1. similiar to common cold
2. brething becomes more labored
3. cough increases
4. wheezing
5. difficulty feeding
6. dehydration