Mid Terms Ibawi Xd Gram Positive Non-Spore Forming Bacilli

Front 1

A gram positive non spore forming bacilli that is primarily an animal pathogen?

Back 1

Listeria monocytogenes

Front 2

Motility of Listeria monocytogenes?

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Tumbling motility at room temperature

Front 3

Screening examination for the identification of Listeria monocytogenes?

Back 3

Wet mount and hanging drop method

Front 4

What is the hemolytic pattern of Listeria monocytogenes?

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Beta hemolytic pattern due to the virulence factor

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Virulence factor of Listeria monocytogenes?

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Listeriolysin O

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Listeria monocytogenes forms the so called_______ umbrella like growth on agar surface SIM media(semi-solid medium)?

Back 6

Inverted Christmas tree

Front 7

To culture Listeria monocytogenes it requieres an increase in ______?

Back 7

Increase in CO2 concentration

Front 8

Selective medium for Listeria monocytogenes?

Back 8

Mc Bride's media

Front 9

Virulence test/ocular test of anton, organism inoculated in the conjunctival sac of rabbit(put on the eyes of the rabbit)?

Back 9

Anton's test

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Positive result of antons test?

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Purulent conjunctivitis or Sore eyes on the eyes of the rabbit

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Diseases caused of Listeria monocytogenes?

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Human and Bovine abortion, Listeriosis

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Mode of transmission of Listeria monocytogenes?

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Ingestion of cheese or coleslaw

Front 13

Cytotoxic and hemolytic toxin that enables Listeria monocytogenes to survive inside a phagocyte?

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Listeriolysin O

Front 14

Listeria monocytogenes is CAMP test?

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Positive and can tolerate cold enrichment

Front 15

Culture media for the CAMP test of Listeria monocytogenes?

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Sheeps blood agar plate

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Type of hemolysis produced of Listeria monocytogenes on CAMP test?

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Blocked type hemolytic pattern

Front 17

How is the camp test of Listeria monocytogenes done?

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The known organism is Rhodococcus equi and the unknown organism is the suspected Listeria monocutogenes

Front 18

Commonly mistaken as Listeria moclnocytogenes?

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Erysipelothrix rhusiopathiae

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How do you differentiate Erysipelothrix rhusiopathiae from Listeria monocytogenes ?

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You perform the H2S test Erysipelothrix rhusiopathiae(+) and Listeria monocytogenes(-) on Triple Sugar Iron media

Front 20

The only producer of Hydrogen Sulfide(H2S) on TSI media?

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Erysipelothrix rhusiopathiae

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A localized skin infection caused by Erysipelothrix rhusiopathiae and an occupational hazard for those handling meat, poultry, and fish?

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Erysipeloid

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Motility of Erysipelothrix rhusiopathiae?

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Non-motile

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What is the colonial appearance of Erysipelothrix rhusiopathiae on gelatin stab?

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Test tube brush appearance/Pipe cleaner brush apearance /bottle brush appearance

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Biological hazard to veterenarians or animal handlers?

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Erysipelothrix rhusiopathiae

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Lactic acid producing organism, non-pathogenic and has little clinical significance

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Lactobacillus acidophilus/Doderlain Bacilli

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A gram positive non spore forming bacteria that is a normal flora of mouth, GIT and vaginal canal?

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Lactobacillus acidophilus

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Media used to culture Lactobacillus acidophilus?

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Tomato juice agar

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Also known as Kleb Loeffler's Bacilli; initially non toxigenic?

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Corynebacterium diphtheriae

Front 29

Demonstrate a side to side arrangent on gram stain?

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Corynebacterium diphtheriae

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Can produce beaded appearance under microscope because it posses metachromatic granules

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Corynebacterium diphtheriae

Front 31

5 culture media used for Corynebacterium diphtheriae

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1. BAP

2. Loefflers coagulated egg medium

3. PAI's coagulated egg media

4. Tinsdale medium

5. CTBA ( Cysteine tellutite blood agar plate)