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24 Cards in this Set
- Front
- Back
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Partition Chrom |
For low molecular weight analyte, ionic compounds, and stationary phase is second liquid that is immiscible into the liquid mobile phase |
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Liquid-liquid partition chrom |
Liquid is held in place by physical adsoprtion |
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Liquid-bonded phase chrom |
Liquid is attached by chemical bonding; compatible with gradient elution |
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Normal Phase Chrom |
-SP prepared from rigid silica with uniform particle size of 2-10 um -polar silica surface without modification |
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Reversed Phase Chrom |
Nonpolar SP packick is modified silica or alumina with rgroup c8 c12 c18 |
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Reversed Phase Chrom |
Nonpolar SP packick is modified silica or alumina with rgroup c8 c12 c18 |
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Increasing adsorption on polar sp |
Alkane, alkyl halide, alkenes, dienes, ethers, esters, ketones, aldehydes, amines, alcohol, phenols, COOH, sulfonic acid |
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Increasing mp polarity |
Hexane, cyclohexane, propanol, thf, ethanol, methanol, acetonitrile, water (Hexane, petroleum ether, cyclohexane, toluene, chloroform, dichloromethane, diethyl ether, ethyl acetate, acetone, 2-propanol, ethanol, methanol, water, acetic acid |
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Increasing elution power |
Hexane, pentane, cyclohexane, toluene, chloroform, dcm, t-butanol, diethyl ether, acetonitrile, 2-propanol, ethyl acetate, acetone, ethanol, thf, methanol, pyridine |
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Separation of relatively polar, water soluble organic compound
Polar solid sp, nonpolar liquid mp |
Adsorption Chrom |
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Measure of solvent adsorption energy |
Eluent strength |
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Molecular sieve Separate molecules of different sizes Sp: porous silica/polymer particles |
Size-exclusion chrom |
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Hydrophilic packings and aq mp For polar compounds |
Gel filtration |
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Molecular sieve Separate molecules of different sizes Sp: porous silica/polymer particles |
Size-exclusion chrom |
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Hydrophilic packings and aq mp For polar compounds |
Gel filtration |
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Hydrophobic packings and non-polar organic mp For less polar compound |
Gel permeation |
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Subset of rpc; easily ionizable species are separated on rpc |
Ion-pair ion chrom |
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Mixture of similar charged ion separated using ion exchange resin |
Ion-exchange ion chrom |
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Contains a suppressor column immediately following the ion-exchange column Suppressor column converts the ionic eluent to non-ionic form form and analyte ions to more highly confucting species species |
Suppressor-based / supressed IC |
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Contains a suppressor column immediately following the ion-exchange column Suppressor column converts the ionic eluent to non-ionic form form and analyte ions to more highly confucting species species |
Suppressor-based / supressed IC |
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Ion supression is unnecessary Used eluent with relatively low conductivity
Suppression of eluent conductivity and enhancement of analyte conductivity is performed electronically by the detector |
Single column w/o supression / unsuppressed IC |
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Contains a suppressor column immediately following the ion-exchange column Suppressor column converts the ionic eluent to non-ionic form form and analyte ions to more highly confucting species species |
Suppressor-based / supressed IC |
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Ion supression is unnecessary Used eluent with relatively low conductivity
Suppression of eluent conductivity and enhancement of analyte conductivity is performed electronically by the detector |
Single column w/o supression / unsuppressed IC |
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Most specific chrom; high selectivity and high resolution |
Affinity Chrom |
