Lesson 1B - Rbc Biology And Preservation

Front 1

- Normal chemical composition and structure of the membrane

- Hemoglobin structure and function

- RBC metabolism

Back 1

What are the three areas for normal erythrocyte survival and function?

Front 2

RBC Rejuvination

Back 2

Process by which ATP and 2,3-DPG levels are restored or enhanced by metabolic alterations

Front 3

Integral Membrane Proteins

Back 3

Proteins that extend from the outer surface and span the entire membrane of the entire cytoplasmic side of the RBC

Front 4

Peripheral Proteins

Back 4

Protein beneath the lipid bilayer, they are located and limited to the cytoplasmic surface of the membrane forming the cytoskeleton

Front 5

Deformability and Permeability

Back 5

What are the two important RBC characteristics?

Front 6

Deformability

Back 6

To remain viable, normal RBCs must also remain flexible, defirmable, and permeable

Front 7

Permeability

Back 7

RBC is freely permeable to water and anions (Chloride and Bicarbonate)

Front 8

True

Back 8

RBC membrane is to cations (Sodium and Potassium) True or False.

Front 9

Oxygen delivery

Back 9

What is the principal purpose of hemoglobin molecule?

Front 10

Left shifted

Back 10

↑Abn Hb

↑pH

↓DPG

↓Temp

↓P30

Front 11

Right shifted

Back 11

↓pH

↑DPG

↑Temp

↑P30

Front 12

2-3 diphosphoglycerate

Back 12

An RBC organic phosphate; it has an important control on hemoglobin for oxygen

Front 13

RBC Preservation

Back 13

To provide viable and functional blood components for patients requiring blood transfusion

Front 14

75% RBC survival

Back 14

An average of more than ______ is required by the FDA for 24-hour post transfusion

Front 15

1 to 6 deg C

Back 15

Storage temperature to maintain optimum viability of the blood

Front 16

ACD-A

Back 16

Acid citrate-dextrose (formula A)

Front 17

CPD

Back 17

Citrate-phosphate dextrose

Front 18

CP2D

Back 18

Citrate-phosphate-double-dextrose

Front 19

CPDA-1

Back 19

Citrate-phosphate-dextrose-adenine

Front 20

ACD-A

CPD

CP2D

Back 20

Has a storage time of 21 days

Front 21

CPDA-1

Back 21

Has a storage time of 35 days

Front 22

42 days

Back 22

Additive solutions extends shelf life pf RBC for up to ____ by adding nutrients

Front 23

True

Back 23

Additive solutions allows for harvesting of more plasma and platelets from the unit. True or False

Front 24

False. Lower

Back 24

RBC concentrate has higher viscosity thus easier to infuse.

Front 25

RBC Freezing

Back 25

Primarily used for autologous units and storage of rare blood types

Front 26

10 years

Back 26

RBC freezing allows long term storage for up to?

Front 27

True

Back 27

RBC freezing causes low residual leukocytes and platelets

Front 28

-65 deg C

Back 28

What is the storage requirements of RBC freezing?

Front 29

High Concentration Glycerol

Back 29

40% weight in volume

Front 30

-65 deg C

Back 30

What is the maximum storage temperature of High Concentration Glycerol?

Front 31

Low Concentration Glycerol

Back 31

20% weight in volume

Front 32

-120 deg C

Back 32

What is the maximum storage temperature of Low Concentration Glycerol

Front 33

-80 deg C

Back 33

What is the initial freezing temperature of high glycerol?

Front 34

-196 deg C

Back 34

What is the initial freezing temperature of low glycerol?

Front 35

Mechanical

Back 35

Type of freezer of High glycerol

Front 36

Liquid Nitrogen

Back 36

Type of freezer of Low glycerol

Front 37

Dry ice

Back 37

Type of shipping requirements of High glycerol?

Front 38

Liquid nitrogen

Back 38

Type of shipping requirements of Low glycerol?

Front 39

Can be thawed and refrozen

Back 39

Effect of changes in temperature in high glycerol

Front 40

Critical

Back 40

Effect of changes in temperature in low glycerol

Front 41

Rejuvesol (enCyte Systems)

Back 41

Only FDA approved rejuvenation solution in the US

Front 42

PIPA

Back 42

Phosphate, Inosine, Pyruvate, and Adenine

Front 43

False. 3 days

Back 43

RBCs stored in the liquid state can be rejuvenated outdate or up to 2 days after outdate. True or False

Front 44

150,000 to 450,000 per UI

Back 44

Normal platelets count

Front 45

9-12 days

Back 45

Platelets are released and circulate approximately ______ as small, disk-shaped with an average diameter pf 2-4 um

Front 46

20 deg C to 24 deg C

Back 46

Platelets are stored at _______ for 5 days with continuous agitation

Front 47

Potential bacterial growth

Back 47

Major concern associated with storage of platelets is the potential for _______