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9 Cards in this Set

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What needs to be done before ABX susceptibility?

Form cultures, identify organism, susceptibility test

What are some types of ABX susceptibility testing?

Broth dilution


Antimicrobial Gradient


Disk diffusion



What is the broth dilution test?

Innoculate same amount of a bacterial isolate into dilutions of antibiotic


Lowest dilution that the bacteria doesn't grow in is the Minimum Inhibitory Concentration


Typically done in 96 well microtitre plates

What is the antimicrobial gradient method?

MIC is determined by the intersection of the organism growth with the strip as measured using the scale on the strip

What is the disk diffusion test?

Measure radius, group as resistant/intermediate/susceptible

Why are different types of bacteria more effectively treated by different types of ABX

Due to intrinsic and acquired resistances

What are intrinsic resistances in bacteria?

Occur naturally


Chromosomally encoded




Reduced permeability to ABX


ABX efflux

What are acquired resistances in bacteria?

Results from a mutation in the DNA


OR acquisition of new DNA that carries resistance




Changes in ABX targets


Modification of targets


Direct modification of the ABX