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10 Cards in this Set
- Front
- Back
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how are polyclonal Ab produced
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Antigen plus adjuvant injected into animal, bled and serum purified for Ab's
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distinguishing features of polyclonal versus monoclonal Ab's
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polyclonal, many B cells produce Ab agains different epitopes on Ag, potential cross reaction
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production of monoclonal Ab
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animal given immunogen, Ab response generated, Ab producing B cell harvested, hybidoma formed with myeloma cell, cultured, Ab taken
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Good points of monoclonal Ab
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one epitope only, little crossreactivity
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flow cytometry outline
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fluorescently conjugated Ab's to desired epitopes used, flow of fluid past lazer, behaviour of refracted light indicated properties of cells
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how is cell size determined by flow cytometry
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forward scatter
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how is cell granularity measured by flow cytometry
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side scatter
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4 layers of ELISA
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purified antigen coats wells, sample (patient serum), anti-human enzyme conjugated (alc phos, HRP) Ab, chromogen (DAB etc)
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how does a quantitative ELISA work
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optical density read against absorption curve of known standard conc
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western blot
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gel electrophoresis of serum, proteins separated, electrotransfer onto membrane, incubation with enzyme conjugated Ab, chromogen
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