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7 Cards in this Set
- Front
- Back
Oxidation-Fermentation (OF) Test |
Determines what catabolic pathway abacterium can use to break down a carbohydrate |
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Catabolic Pathways |
1. Aerobic Respiration 2. Fermentation |
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Aerobic Respiration |
Oxidationpathway - O2 final electronacceptor - Oxygenenvironment required - smallamount ofacidicintermediate compoundsproducedbody |
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Fermentation |
Fermentationpathway - Organic moleculefinal electronacceptor - Occurs in the presence or absence ofO2 - largeamounts of acidiccompoundsproduced |
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Two important media components |
1. A carbohydrate (e.g.,glucose, sucrose, or lactose) [OF-glucose media used in this exercise] 2. The pHindicatorBROMTHYMOLBLUE |
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Phenol Red Broth Test |
usedto determine whatcarbohydrate abacterium can ferment |
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Nitrate Reduction Test |
todetermine if an organism can use nitrate for either denitrification or ammonification |