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7 Cards in this Set

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Oxidation-Fermentation (OF) Test

Determines what catabolic pathway abacterium can use to break down a carbohydrate

Catabolic Pathways

1. Aerobic Respiration


2. Fermentation

Aerobic Respiration

Oxidationpathway


- O2 final electronacceptor


- Oxygenenvironment required


- smallamount ofacidicintermediate compoundsproducedbody

Fermentation

Fermentationpathway


- Organic moleculefinal electronacceptor


- Occurs in the presence or absence ofO2


- largeamounts of acidiccompoundsproduced

Two important media components

1. A carbohydrate (e.g.,glucose, sucrose, or lactose) [OF-glucose media used in this exercise]


2. The pHindicatorBROMTHYMOLBLUE

Phenol Red Broth Test

usedto determine whatcarbohydrate abacterium can ferment

Nitrate Reduction Test

todetermine if an organism can use nitrate for either denitrification or ammonification