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29 Cards in this Set
- Front
- Back
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Where are chloroplasts found?
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in mesophyll cells
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What is found in chloroplast isolation medium?
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buffer
sucrose, NaCl MgCl2 (keeps membrane structure function) |
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What do you save first? the supernatant or the pellet? which one do you save the second time?
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Save the supernatant the first time
save the pellet the second time. |
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how does photosystem ii contribute to photosynthesis?
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splits water; produces oxygen in the process
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What color is DCPIP when it is oxidized? what about when it's reduced?
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blue/colorless
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What is the role of DCPIP?
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mimics NADP+
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What is the spectrum of wavelength used for the chloroplasts?
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400-700nm
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What light do chloroplasts absorb?
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Blue light
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What is most important about the cohesion-tension theory?
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If the stomata are open & the air is dry, water will move out.
then water will move from the ground up throughout the plant. |
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If stomata are open, is it turgid or flaccid?
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turgid
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Do monocots or eudicots have a fibrous tap root? what does the other one have then?
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monocots have a fibrous tap root
eudicots have a fibrous root system |
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What is in the cortex inside the root hair of epidermal cells?
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ground/storage cells
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The xylem is made up of
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dead cells that transport water and dissolved minerals to the upper part of the plant
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the phloem is made up of
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live specialized cells to transport sugars and other dissolved organ solutes
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Which one has vascular bundles arranged in a circle?
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eduicot stem
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Which one has a parallel orientation of veins?
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monocots; eudicots have a net vein pattern
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What happens in the zone of differntiation?
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different kinds of tissues are distinguishable
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What species of spinach leaves are we using?
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Spinacia oleracea
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What chlorophyll does photosystem II use?
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p680
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What is transpiration?
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the loss of water
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What is the pH of the cytoplasmic matrix?
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7.4
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What is the role of inorganic phosphate at a concentration of 0.05M and at a pH of 7.4?
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acts as a buffer (resists changes in pH); acts like the cytoplasmic matrix.
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What color does safranin stain? What does it stain?
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red;
nuclei and lignin |
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What color does crystal violet stain? what does it stain?
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violet; starch, mucilage, middle lamella
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What color does fast green stain? What does it stain?
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green; cellulose cell walls
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What does gold orange stain?
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structures not covered by other stains.
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Is the oxidized or reduced form of biological molecules more stable?
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reduced form
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What is the role of Chelator?
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bind to metal ions that would otherwise interact with and damage an organelle
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What is the best temperature to store cells removed from their environment?
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0-4 (refrigerator)
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