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19 Cards in this Set
- Front
- Back
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indole
pyruvic acid ammonia |
What are the end products of tryptophan hydrolysis?
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Medium (plural: media)
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a food or nutrient source for culturing microorganisms. To support the growth of living cells it must contain all the basic requirements of cellular life: water, a carbon source, nitrogen, sulfur, phosphorus, and various metallic ions, e.g. : K+, Na+ ...
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growth factors
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In addition, some organisms require special ________ ______ (any essential component, such as certain A.A. or vitamins, which the organism can not synthesize on its own.
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Non-synthetic media
Synthetic or chemically defined media. |
What are the 2 major categories of media used for routine culture of organisms?
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Non-synthetic media
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A.k.A complex media
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Synthetic or chemically defined media.
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composed of known qualities of chemically pure compounds, prepared to exact specifications.
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Non-synthetic media
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contains extracts of plant and animal tissues and its exact composition is not known.
e.g. : milk, beef extract, plant infusions ... |
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Solid media
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Is used to observe the appearance of colonies, and for pure culture isolation. There is a solidifying agent that is added.
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Agar
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a solid media
a polysaccharide extracted from a marine red algae, has unique properties that make it very useful as a solidifying agent. only a few organism can degrade this solid. liquifies at 100'C cooled at 40'C before it become at solid. allows for incubation at high temps w/out liquefying. 50'C is at a liquid state. |
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Liquid/broth
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is used to grow up large numbers of an organism, determine an organisms ability to ferment cetain sugars and various other biochemical tests.
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Semisolid
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Contains less solidifying agent than solid media and has a jellylike consistency.
used in motility studies, O2 requirements and as transport media |
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by its consistency or viscosity.
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Media can also be defined by its ___________ or viscosity.
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TSA and NA
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What are the general purpose media that we use in this class?
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EMB
MSA |
Selective and Differential
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PEA
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Selective
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BA
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Enriched and differential
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Selective
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Contains dyes or toxic substances which discourage the growth of certain microbes, but supports the growth of others.
e.g. EMB and PEA |
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Differential
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Different microbes produce distinctive colonies (color, texture, etc.) on this type of media.
e.g. EMB |
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Enriched
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Growth substances are added to give more luxurious growth or allow for growth of organisms that can not grow on general purpose media.
e.g. BA |
