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19 Cards in this Set

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indole
pyruvic acid
ammonia
What are the end products of tryptophan hydrolysis?
Medium (plural: media)
a food or nutrient source for culturing microorganisms. To support the growth of living cells it must contain all the basic requirements of cellular life: water, a carbon source, nitrogen, sulfur, phosphorus, and various metallic ions, e.g. : K+, Na+ ...
growth factors
In addition, some organisms require special ________ ______ (any essential component, such as certain A.A. or vitamins, which the organism can not synthesize on its own.
Non-synthetic media

Synthetic or chemically defined media.
What are the 2 major categories of media used for routine culture of organisms?
Non-synthetic media
A.k.A complex media
Synthetic or chemically defined media.
composed of known qualities of chemically pure compounds, prepared to exact specifications.
Non-synthetic media
contains extracts of plant and animal tissues and its exact composition is not known.

e.g. : milk, beef extract, plant infusions ...
Solid media
Is used to observe the appearance of colonies, and for pure culture isolation. There is a solidifying agent that is added.
Agar
a solid media

a polysaccharide extracted from a marine red algae, has unique properties that make it very useful as a solidifying agent.

only a few organism can degrade this solid.

liquifies at 100'C

cooled at 40'C before it become at solid.

allows for incubation at high temps w/out liquefying.

50'C is at a liquid state.
Liquid/broth
is used to grow up large numbers of an organism, determine an organisms ability to ferment cetain sugars and various other biochemical tests.
Semisolid
Contains less solidifying agent than solid media and has a jellylike consistency.

used in motility studies, O2 requirements and as transport media
by its consistency or viscosity.
Media can also be defined by its ___________ or viscosity.
TSA and NA
What are the general purpose media that we use in this class?
EMB
MSA
Selective and Differential
PEA
Selective
BA
Enriched and differential
Selective
Contains dyes or toxic substances which discourage the growth of certain microbes, but supports the growth of others.

e.g. EMB and PEA
Differential
Different microbes produce distinctive colonies (color, texture, etc.) on this type of media.

e.g. EMB
Enriched
Growth substances are added to give more luxurious growth or allow for growth of organisms that can not grow on general purpose media.

e.g. BA