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23 Cards in this Set

  • Front
  • Back

FTM useful for studying aero tolerance of given microbe

Fluid Thioglycollate Medium


Excellent for cultivating obligate anaerobes and Microaerophiles

Microorganisms with complex nutritional requirements and specific growth conditions

Fastidious microbes

Reazurin

Dye that turns pink when it is oxidized by oxygen

Psychrophile microorganisms

Grow below 20 degree C

Psychrtroph

Grow between 0-30 degrees C

Mesophile

Grow between 15-45degrees C

Thermophile

Grow above 40 degrees c

Extreme thermophile

Grow above 80 degrees C

What is the differential agent in starch hydrolysis test

Differential agent is Starch used to identify presence of a=amylase and oblige 1 and 6 glucosidase

Indicator for starch hydrolysis test is

Iodine this is added after incubation

In a starch hydrolysis test the presence of starch is indicated by ?

Blue black or dark brown color. Clearing around colony means absence of starch

Casein Hydolysis test differential agent is

Differential agent is milk. Used to identify presence in of cases see which hydro lines casein( primary protein in milk)

Casein Hydolysis test differential agent is

Differential agent is milk. Used to identify presence in of casease which hydrolizes casein( primary protein in milk)

Casein hydrolysis test indicator is?

Indicator is clearing of the “white” color produced by the milk protein casein

Lipid hydrolysis testdifferential agent is

Tributyrine oil used to identify the presence of lipases that hydrolysis lipids or tryglicerides for transport into cells

Lipid hydrolysis test indicator is

Indicator is clearing of the cloudiness originally produced by the presence of oil in the agar

Gram positive, groups of cocci, salt tolerant so isolated on MSA, cause food poisoning, toxic shock, pink eye, etc., coagulase positive

Staphylococcus aereus.

Anaerobic, produces endospores, causes intestinal hemorrhaging


Clostridium sporogenes

Aerobe, motile, fruity smelling, produces blue-green pigment on culture plates, has phagocyte resistant slime layer, can infect any part of the body

Pseudomonas aeruginosa

Gram negative rod, opportunistic pathogen infecting heart valves, CNS, GI, bones, joints ; isolated on Pseudomonas isolation agar

Pseudomonas aeruginosa

5. Gram-negative rod.. common resident of digestive tract, important for health because it produces vitamins K and B complex, indicator bacterium for fecal contamination

Escherichia coli

Causative agent of common UTI(urinary tract infection), Montezuma’s revenge, food poisoning, strain O157:H7 enterohemohrrahgic that produces a toxin

Escherichia Coli

. Gram positive coccus, commonly found in digestive tract, causes endocarditis, cystitis, wound infections, risky to patients with urinary or intravascular catheterization

Enterococcus faecalis