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10 Cards in this Set
- Front
- Back
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Oxidase Test |
-Medium: Tryptose agar or nutrient agar slants -Substrate: Oxidase Reagent (Dimenthyl-p-phenylene) diamine hydrochloride) Enzyme:Oxidase Enzyme Indicator/Reagent: Oxidase Reagent (+)Dark purple (-) No color change End product: Indoph |
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Synthetic Media |
-Chemically defined -Can be reproduced wit precision -ie Nutrient agar |
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Living media |
1)Media composed of living cells 2)Not chemically defined 3)Ie. Tissue cultures cells for viruses 4)Lab Animals and plants |
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Isolation Media |
Duplicate Microbes natural environment, optimal requirements for growth -Rich media supplies optimal growth requirements -Ie. Sabouraud Agar pH 5.6 Mold and yeast -Trypticase soy agar Bacteria pH 7.2 -Zobell 2216 Marine bacteria 3%NaCl |
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Maintenance Media |
-Maintain Minimal Growth requirements -Slow growth because not the optimal conditions are met -Nutrient agar |
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Enrichment Media |
Rich Media -Required for for growth of Fastidious Microbes (Streptococcus Species) -ie Bood agar |
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Differential Media |
Differentiates groups or types organisms by a specific chemical reaction usually observed by a color change due to the presence of an indicator -Does not prevent growth (all types of microbes grow) -Chemical reaction=results in color change - |
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Alpha Hemolysis (diff Media) |
- partial incomplete Hemolysis Green color -Alpha hemolysin;exo/extracellular enzyme |
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Beta Hemolysis |
-Complete Hemolysis, Clear -Beta hemolysin exo/extracellular enzyme |
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Gamma Hemolysis |
-No Hemolysis No change in Media -gamma hemolysin; exo/extracellular enzyme |
