Reading...
Play button
Play button
Use LEFT and RIGHT arrow keys to navigate between flashcards;
Use UP and DOWN arrow keys to flip the card;
H to show hint;
A reads text to speech;
19 Cards in this Set
- Front
- Back
|
MacConkey and EMB agar
|
Selective gram - agar inhibitory gram +
Lactose fermentation indicator |
|
|
MacConkey agar
|
yellow agar used in gram - id
inhibitory by bile salts + lactose ferementation = pink - lactose ferementation = no change |
|
|
EMB agar
|
brownish agar used in gram - id
inhibited by methylene blue rapid lactose ferm = metallic green reg lactose ferm=nucleated colonies, black nucleus no lactose ferm= no color change |
|
|
TSI slant
|
red/pink slant used in gram - id
triple sugar iron agar stab and streak method contains a slant and a butt |
|
|
TSI slant
|
aerobic portion
shows if we have lactose ferm + gives us a color change from red to yellow |
|
|
TSI butt
|
anaerobic portion
shows us if we have glucose ferm + changes agar color from red to yellow |
|
|
TSI "black" butt
|
hydrogen sulfide and iron production produces a black precipitate in the butt
also is a positive for glucose ferm; "black butt also means that you had a yellow butt" |
|
|
Gas production indicator
|
seen as cracks or bubbles in the TSI slant
|
|
|
Tryptone broth
|
day 1: inoculate
day 2: evaluate by adding Kovac's reagant (indicator of indole) |
|
|
What is indole?
|
Indole can be produced by bacteria as a degradation product of the amino acid tryptophan. It occurs naturally in human feces and has an intense fecal odor. At very low concentrations, however, it has a flowery smell, and is a constituent of many flower scents (such as orange blossoms) and perfumes. It also occurs in coal tar.
|
|
|
Positive indole test
|
after the addition of Kovac's reagant in gram - tryptone broth = bright pink layer on top
|
|
|
Negative indole test
|
after the addition of Kovac's reagant in gram - tryptone broth = no color change (yellowish color of Kovac's)
|
|
|
Gram +
Staph tests |
Manitol salt agar
DNase agar Brain heart infusion |
|
|
Manitol salt agar
|
pink/red agar Staph test
halophiles/facultative halophiles differentiate on org ability to ferment manitol + manitol ferm = bright yellow - manitol ferm = pink |
|
|
DNase agar
|
turquoise agar; organisms ability to hydrolize/ingest DNA.
Enzyme response in DNase. + DNase=clear halos around growth - DNase=no change |
|
|
Brain Heart Infusion
|
staph test
coagulase test + coagulase = clot - coagulase = no clot |
|
|
Bile Esculin Slants
|
strep test-brown slant
hydrolyzes esculin +esculin = black - esculin = no change, brown |
|
|
Phenol Red Manitol
|
strep test-pink/red slant
ability to ferment manitol + manitol = yellow - manitol = no change, pink |
|
|
Catalase Test
|
catalase: enzyme that catalyzes reaction; breakdown H2O2 into water and oxygen
+ catalase= bubbles - catalse= no bubbles Staphs are catalase positive |
