Lab Exam #2 T/f

Front 1

The bacterial endospore is more resistant to adverse environmental conditions than the vegetative cell.

Back 1

T

Front 2

Bacteria form spores as a survival mechanism in response to adverse environmental conditions.

Back 2

T

Front 3

Most bacteria capable of forming spores are organisms of the genera Bacillus and Clostridium.

Back 3

T

Front 4

The spore is the dormant, or resting phase, of the bacterial cell.

Back 4

T

Front 5

The detection of endospores is useful in identifying bacteria.

Back 5

T

Front 6

The size and location of the spore within the vegetative cell depend on physical and chemical conditions in the environment during sporulation

Back 6

F

Front 7

Endospores can be visualized only by the Schaeffer-Fulton spore stain.

Back 7

F

Front 8

Safranin is the primary stain in the Schaeffer-Fulton technique.

Back 8

F

Front 9

Because the spore coat is so resistant, the stained spore tends to decolorize quickly if the smear is washed too much before the counterstain is applied.

Back 9

F

Front 10

In a Schaeffer-Fulton spore stain prepariation, spores appear as small, green ovals or spheres, and vegetative cells are red

Back 10

T

Front 11

Volutin granule is another term for metachromatic granule

Back 11

T

Front 12

Metachromatic granules are found only in bacteria of the genus Corynebacterium.

Back 12

F

Front 13

Diphtheroid bacteria cause diptheria

Back 13

F

Front 14

Metachromatic granules have an affinity for basic dyes and stain more intensely than the surrounding cytoplasm

Back 14

T

Front 15

The location of metachromatic granules within the bacterial cell can be a useful aid to species differentiation

Back 15

T

Front 16

Albert's differential stain is often call Albert's diptheria stain

Back 16

T

Front 17

Corynebacterium xerosis is a diphtheroid bacterium

Back 17

T

Front 18

One should heat-fix a smear intensively before staining for metachromatic granules.

Back 18

F

Front 19

Bipolar staining results when metachromatic granules are located mainly at the ends of a bacterium.

Back 19

T

Front 20

Corynebacterium diphtherieae display a characteristic palisading cell arrangement

Back 20

F

Front 21

Lipid and glycogen can be cytoplasmic inclusion in bacteria.

Back 21

F

Front 22

Lipid and glycogen can be cytoplamsic inclusion in bacteria

Back 22

T

Front 23

Metachromatic granules become more distinct when all available nutrients are depleted but starvation has not yet occured.

Back 23

T

Front 24

Corynebacterium pseudodiphtheriticum does not form metachromatic granules

Back 24

T

Front 25

The term diphtheroid describes a diphtheria-like, nonpathogenic species of Corynebacterium

Back 25

T

Front 26

Disinfectants always remove all bacteria from an object

Back 26

F

Front 27

Antiseptic is a synonym for disinfectant

Back 27

F

Front 28

The presence of blood serum interferes with the antimicrobial action of disinfectants

Back 28

T

Front 29

Disinfectants are used as gargles

Back 29

F

Front 30

Carbolic acid used as a germicide in the operating room was the beginning of aseptic surgery.

Back 30

T

Front 31

Confluent growth is a thick growth of bacteria over the entire surface of the medium.

Back 31

T

Front 32

The zone of inhibition is the clear zone around an antimicrobial agent in which no bacteria grow

Back 32

T

Front 33

Room-temperature sterilization implies removal of all microbes form an object by using a disinfectant

Back 33

T

Front 34

Disinfectants are all equally efffective against most microbes

Back 34

F

Front 35

Cutting instruments, thermometers, and plastic materials are best sterilized by autoclaving.

Back 35

F

Front 36

The lower the phenol coefficient, the stronger the disinfectant.

Back 36

F

Front 37

Louis Pasteur introduced aseptic surgery by using a disinfectant

Back 37

F

Front 38

By-products of metabolis produced by microbes are useless to all cells.

Back 38

F

Front 39

If an antibiotic is effective in a sensitivity test against an organism that causes an infection, the same antibiotic will always stop the infection in the patient if it is cuased by the same organism.

Back 39

F

Front 40

Chemotherapeutic agents are synthesized in vitro

Back 40

T

Front 41

There are 100 mm in a centimeter

Back 41

F

Front 42

A good antibiotic is toxic to all cells

Back 42

F

Front 43

Gram-positive bacteria are normally more sensitive to antibiotics than Gram-negative bacteria

Back 43

T

Front 44

Drug-resistant mutants often arise because the patient does not take all the antibiotic prescribed.

Back 44

T

Front 45

To determine the size of the zone of inhibiton, it is customary to measure its radius.

Back 45

F

Front 46

The Staphylococcus aureus strain used in the laboratory to evaluate susceptibility is likely to cause a nosocomial infection

Back 46

F

Front 47

Lypolysis results from lipase activity on complex carbohydrates

Back 47

F

Front 48

Starch is a complex carbohydrate

Back 48

T

Front 49

Amylase is a carbohydrase

Back 49

T

Front 50

Some endoenzymes are active outside the cell

Back 50

F

Front 51

Anabolic enzyme reactions result in biosynthesis

Back 51

T

Front 52

Catabolic enzyme reaction result in cleavage of the substrate molecule into smaller molecules.

Back 52

T

Front 53

Catabolic enzyme reactions result in cleavage of the substrate molecule into smaller molecules.

Back 53

T

Front 54

Catabolic enzyme reactions produce energy

Back 54

T

Front 55

peptides and amino acids are end products of protein hydrolysis

Back 55

T

Front 56

Iodine is a positive test for protein

Back 56

F

Front 57

Tributyrin is a fat

Back 57

T

Front 58

Disaccharides are too large in molecular size to pass through the cell membrane

Back 58

F

Front 59

The enzymatic activity of caseinase is manifested by liquefactin of the skim milk agar

Back 59

F

Front 60

Lipase degrades the fat molecule to glycerol and fatty acids

Back 60

T

Front 61

There are 100 mm in a centimeter

Back 61

F

Front 62

A good antibiotic is toxic to all cells

Back 62

F

Front 63

Gram-positive bacteria are normally more sensitive to antibiotics than Gram-negative bacteria

Back 63

T

Front 64

Drug-resistant mutants often arise because the patient does not take all the antibiotic prescribed.

Back 64

T

Front 65

To determine the size of the zone of inhibiton, it is customary to measure its radius.

Back 65

F

Front 66

The Staphylococcus aureus strain used in the laboratory to evaluate susceptibility is likely to cause a nosocomial infection

Back 66

F

Front 67

Lypolysis results from lipase activity on complex carbohydrates

Back 67

F

Front 68

Starch is a complex carbohydrate

Back 68

T

Front 69

Amylase is a carbohydrase

Back 69

T

Front 70

Some endoenzymes are active outside the cell

Back 70

F