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15 Cards in this Set
- Front
- Back
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Phenylethylalcohol Agar |
selective media; allows grow of gram(+) cells. Phenylethylalcohol is toxic to gram(-) cells |
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Mannitol Salts Agar |
selective/differential media; salts found in the media allow hypertonic growth; phenol red found in the media will change color in regards to acidity (red(-) yellow(+) |
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MacConkey Agar |
selective/differential media; crystal violet inhibits growth of gram(+) growth, Neutral red shows the PH(acidity) of bacteria and presence of lactose, shows the cells ability to ferment |
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Eosin-Methylene Blue Agar |
Selective/differential media; EMB inhibits gram (+) growth. EMB also shows if a bacteria is a lactose &/or sucrose fermenter ; Lactose fermenter turned green |
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Blood Agar |
Differential media; hemolysis (break down of hemoglobin; red blood cells) allows us to compare bacteria |
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Alpha Hemolysis Beta Hemolysis Gama Hemolysis |
alpha- partial breakdown beta- complete breakdown gama- no breakdown |
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Catalase Test |
bacteria that are positive for a catalase will break down H202 producing oxygen and water forming a foam or bubbling |
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Starch hydrolysis Test |
Starch is a carbohydrate; test shows the ability of a bacteria to break down starch. adding iodine will change the color of the agar only when starch is no longer present |
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Egg Yolk Agar |
tests for the ability to break down lipids/proteins. positive test will show a clearing in the plate while a negative plate will show no change |
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Gelatinase Test |
detects the ability to break down gelatinase for energy use. Positive test will turn the agar from a solid to a liquid |
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Phenol Red Test |
detects the ability to break down sugars. when a bacteria ferments CO2 and H2O a produced. The broth will stay red when fermentation does not happen. Detects acidity (PH) the durham tube is used to collect CO2 that is produced |
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Triple Sugar Iron Agar (TSIA) |
tests for production of H2S, metabolism of sucrose &/or lactose, production of gas during fermentation, production of acid during during fermentation |
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Urease Test |
tests for the presene of urease in a cell. PH increases the broth will turn red |
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SIM (sulfide, indol, motility) |
tests for motility, the presence of indol, and production of Sulfide. positive test for indol will show a red band at the top of the tube, positive test for motility will show turbidity, positive test for sulfide will have a black precipitate
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Methyl Red Test |
Tests for the presence |
