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7 Cards in this Set
- Front
- Back
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Hemolysis Test:
-alpha -beta -gamma |
-alpha hemolytic: partial/incomplete lysis of RBCs causing a greenish halo around colony
-beta: complete lysis of RBS causing a clear halo -gamma: non-hemolytic |
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Fermentation Assay
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-if phenol red solution is red, pH is >7 = no fermentation
-if PR sol. is yellow, pH is <7 indicating bact has fermented carbohydrate broth into acid |
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Coagulase Test
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if mix bacteria with fibrin and clotting occurs, bact has coagulase enzyme
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Catalase Test
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if mix H2O2 in bacteria culture and water and bubbles arise, bact has catalase enzyme
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Serological Characteristics
-Define? -3 Examples? |
-def: use Abs to detect antigens or use antigens to detect Abs (indirect evidence of infection)
-ELISA -western blot -radioimmunoassay |
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What is the Best Evidence Rule?
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It requires that a party seeking to prove the content of a writing (includes films, photos, X-Rays and recordings) must either:
[1] produce the original document or [2] account for the absence of the original |
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Mannitol Salt Agar (MSA)
-Purpose? -Technique? |
-distinguish S. aureus from other staph species and micrococcus species
-high salt: no micrococcus can grow -if bact can ferment mannitol, phenol red will turn yellow bc pH is <7 (S. aureus is +) |
