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25 Cards in this Set
- Front
- Back
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Membrane filter method pore size? normal size of bacterial cell? Used for?
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Filter size= 0.45 U
bacterial cell= 1 U Used for enumerating fecal choliforms |
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M-FC Broth at what incubation T? Which survives and what doesn't?
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Use of 44.5 C incubation. Ecoli survives, enterobacter does NOT (not unique to colon). Eliminated at 43 C
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What is MPN and used for?
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Most Probable Number. A statistical range of # of chliforms found by seeing # broth tubes showing gas.
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Limitations of MPN? Advantage?
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Does not detect total number of bacteria in water. Does not locate noncholiforms like Salmonella. Shows presence and quality of choliforms
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What is tested in Presumptive water test?
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Lactose broth inoculated with water samples. Gas in Durham tubes show fecal contamination by coliforms and MPN can be found.
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What is tested in the Confirmed water test?
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Gas at 37o can be fecal coliform (metallic green sheen) or non-fecal coliform. Streak onto EMB. Enterobacter spp. show red, non lactose fermentors are colorless
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What is tested in the Completed water test?
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Use BGLB (brilliant green lactose-bile), then subculture onto EMB. Metallic green sheen or gas in lactose broth
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name 3 non coliform lactose - micoorganisms
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Salmonella, Shigella, Proteus
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In Kirby Bauer Analysis, name sizes of zone of clearing for most effective, intermediate, and very sensitive
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<14 mm = starts to be effective
15-17mm = intermediate >18mm = very sensitive |
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what agar is used in Kirby Bauer test?
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Meuller-Hinton agar
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Most effective antibiotic against S.A. +? least? reasonable?
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Most effective= Erythromycin
Least effective= Cloxacillin Reasonable= Sulfadiazine |
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Which antibiotic has no effect on Gm(-)? how can it be effective? What is effective on gm(-) ?
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PCN has no effect on gm (-). Can be if modified. Ampicillin is effective.
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What makes blue pigment & is next big bug in medicine?
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Pseudomonas Aurogenosa. Makes blue pigment "pyocyanin."
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Order of effectiveness of 3 disks in 3 separate plates for S.A.?
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Phenol > Iodine > Formaldehyde
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Order of effectiveness of 3 disks in 3 separate plates for P.A.?
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Iodine > Phenol > Formaldehyde
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Optimum T of E.coli? No growth T? What -phyle type?
Geobacillus growth at? |
37 C optimal growth. No growth at 5 C.
E.C. is a neutraphile. Geo. B. slight growth at 55 C = Thermophile. |
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Color & T for Serratia Marcescens?
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Purple & growth at 25 C.
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S.C. is what and only grows at what ph?
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S.C. is fungus, grows only at pH 7.9
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What is halophilic? Non-halophilic bacteria? Halotolerant bacteria?
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Halophilic: requires salt to grow.
E.C. is non-halophilic Staph is halotolerant |
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Strict anaerobe? Facultative?
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Psuedomonas aeroginosa is stric anaerobe. E.coli is facultative.
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What is sensitive to UV light? Non-clear zone after UV is what?
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E.C. Not clear= mutant form
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What one test differentiates Salmonella from Proteus?
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Salmonella is urease - and Proteus is urease +
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What plates are selective? Differential? Both?
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PEA is selective. BA is differential.
DES and EMB are differential & selective |
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Why isn't E.C. harmful inside us?
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Needs hemorragic D from outside source
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What was used for Gm (+) pyogenic cocci?
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Throat/Nose/Fomite swabs into M-Staph. broth and BA plate.
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