Lab 19 Images For Path Exam 3 (Laegreid)

Front 1

Pig spleen

Back 1

Multifocal granulomatous splenitis (tuberculosis)
Caseous centers surrounded by macros, lymphos, and connective tissue

Front 2

cow lymph node

Back 2

Locally extensive granulomatous lymphadenitis (tubercular)
Node is destroyed. All caseous debris encapsulated by fibrous tissue.

Front 3

cow omentum

Back 3

Small tubercles caused by systemic tuberculosis.
Probably composed solely of macros

Front 4

chicken viscera

Back 4

Multple tubercles all over viscera

Front 5

deer lung

Back 5

Granulomatous pneumonia
Has central mineralization and multinuc'd giant cells --> typical of mycobact.

Front 6

Chicken

Back 6

Hjarre disease (coligranuloma)
necrotic center surrounded by fibrous band with macros and lymphos

Front 7

Cow intestine

Back 7

Johne's Disease:
thickening of int. ruggae by lots of epith. macros in the mucosa and submucosa

Front 8

sleep ileum

Back 8

Macro aggregation in ileum of sheep with johne's

Front 9

Cow with Actinomycosis

Back 9

Sulfur granule surrounded by macros

Front 10

Dog thorax

Back 10

Pyogranulomatous pleuritis caused by nocardia (Nocardiosis)
Sanguinopurulent exudate (yellow)
Sulfur granules (green)
Also, masses of confluent granulomas could be seen.

Front 11

Cow mammary gland

Back 11

Chronic pyogranulomatous mastitis (Nocardiosis)
The red strands are the bacteria (modified acid-fast stain)

Front 12

Dog Lungs

Back 12

Blastomycosis with miliary (green) and large (blue) granulomas all over lungs

Front 13

dog lungs

Back 13

Blastomycosis again
The granulomas look somewhat like abscesses but are less discrete

Front 14

dog lungs

Back 14

Blastomycosis again
The granulomas look somewhat like abscesses but are less discrete

Front 15

Dog Lungs
Exudate
What cells do you see?

Back 15

Blastomycosis (histology)
Cellular exudate:
Microbe cells (blue)
Some inside macros (green)
Neutrophils (red)

Front 16

Dog Lung

Back 16

Histoplasmosis
cells inside macros

Front 17

Silver stain of dog spleen

Back 17

Histoplasmosis
Microbes found inside macros
(Silver stains the capsules)

Front 18

Cow
What is this?

Back 18

No, it's not a raw oyster
It's a pyogranulomatous lesion within a lymph node caused by coccidiodomycosis.

Front 19

What are these?

Back 19

Endospores of Coccidiodes immitis (without the outer sporocyst wall)
These would be found within a granulomatous or pyogranulomatous lesion.

Front 20

What are these?

Back 20

Endospores of Coccidiodes immitis (without the outer sporocyst wall)
These would be found within a granulomatous or pyogranulomatous lesion.

Front 21

What are these?

Back 21

Endospores of Coccidiodes immitis (without the outer sporocyst wall)
These would be found within a granulomatous or pyogranulomatous lesion.

Front 22

What the hell is going on here?

Back 22

This is a granulomatous lesion eroding the skull caused by Cryptococcus. It originates as a nasal sinus infection.

Front 23

Horse brain

Back 23

Cryptococcus again
The large clear spaces are the capsules of the yeast cells. They inhibit immune response and phagocytosis, so the cellular response is surprisingly weak in this picture.

Front 24

Cat brain

Back 24

Meningeal cryptococcosis.
Again, the clear space is from this fungus' thick capsule.

Front 25

Chicken Air Sac and Mesentery

Back 25

The filamentous growth is from Aspergillus. The underlying tissue will be induced to form granulomatous or pyogranulomatous lesions.

Front 26

Chicken Lungs

Back 26

Multifocal Aspergillosis

Front 27

Horse guttural pouch
What are the brown spots

Back 27

They are granulomatous nodules caused by Aspergillosis

Front 28

Chicken Air Sac
Silver Stain

Back 28

Aspergillus mycelia growth with macrophages at the base of the growth

Front 29

Chronic locally extensive ulcerative and pyogranulomatous dermatitis.
Cutaneous Blastomycosis
What will you see?

Back 29

These cells are seen in the exudate

Front 30

Chronic (multi)focal granulomatous pneumonia.
Pulmonary Histoplasmosis
What will you see in the exudate?

Back 30

This here's what y'all'll see.

Front 31

Chronic diffuse granulomatous splenitis
Splenic Cryptococcosis
What do the microbes look like?

Back 31

Thick capsule

Front 32

Chronic locally extensive pyogranulomatous dermatitis
Cutaneous Coccidiomycosis

Back 32

FYI, the sporocyst (containing the endospores) illicits a granulomatous response, while the endospores, when released, illicit a suppurative response.

Front 33

Cow mammary gland

Back 33

Pyogranulomatous mastitis
The filaments are the nocardia bact. cells

Front 34

Dog Lung
Mucicarmine stain

Back 34

Pulmonary cryptococcosis
The mucin in the capsule of C. neoformans stains red so you can visualize it.

Front 35

Actinobacillosis
Massive granuloma from cow abomasum with foci of suppuration (black arrows)
What is within these suppurative foci?

Back 35

Sulfur granules from the bacteria

Front 36

Dog gingival nodule

Back 36

Sections of tissue heavily infiltrated by macrophages (granulomas) with suppurative lesions at the center of them (neutrophils surrounding pieces of plant fiber)

Front 37

Cow colon
Macrophage infiltration of the lamina propria and submucosa
Why?

Back 37

Mycobacterium Paratuberculosis (Johne's disease)
Ziehl Nielsen stain reveals them inside the macrophages

Front 38

Dog lung
Severe diffuse inflammation with alveolar consolidation.
Fibrinopurulent granulomatous exudate.
See something in macrophages (green) What?

Back 38

Toxoplasmosis
See the tachyzoites with Giemsa stain