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53 Cards in this Set
- Front
- Back
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What is the purpose of the adjustment system?
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Raises and lowers the body tube for focusing on the specimen
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What are the objective lenses of a microscope?
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1)Low Power...10X...Green
2)High/Dry....40X...Yellow 3)Oil........100X...Red/Black |
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What are parts of the Adjustment system of a microscope?
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1)Coarse adjustment
2)Fine adjustment 3)Interpupillary distance knob |
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Describe the Ocular Lenses of a microscope
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Lenses through which the viewer uses to observe the specimen.
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What type of cloth should be used to clean a microscope?
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Lint-free cloth
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TRUE or FALSE
The coarse adjustment knob can be used on all objectives. |
FALSE
ONLY on low power objective |
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What is the quantity and unit of measure of the Unopette system?
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20 lambda (uL) |
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Describe the body tube of the microscope.
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tube through which light passes from the objectives to the ocular lenses
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When onboard ship, what cleaning and maintenance instructions would be used for operating a microscope?
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PMS-Preventive Maintenance
System/Schedule MRC-Maintenance Report Card |
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What is the fine adjustment used for?
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Exact focus on the specimen
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What is the minimum and maximum amount of time that the dilution can stand for?
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At least 10 min. but no more than 3 hours. |
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What is the coarse adjustment used for?
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Initially focus on specimen
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When can alcohol be used to clean a microscope?
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NEVER |
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The WBC Count is performed to determine how many WBC's in a sample of blood?
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Per cubic millimeter |
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What are the parts of the unopette system?
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1)Reservoir
2)Pipette 3)Pipette shield |
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How long should the specimen of blood be mixed for prior to diluting in the unopette system?
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1 minute |
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What are normal WBC values for adult males and females?
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4000 to 11,000 WBC/mm3 |
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Define Leukopenia
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Abnormally decreased WBC count
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How many times should the dilution of blood and acid in the reservoir be inverted to completely mix the dilution?
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10-15 times |
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What is the Neubauer Hemocytometer used for?
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WBC Counting
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What is used to clean the objective and ocular lenses?
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Lens paper and Lens cleaner
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What is the interpupillary distance control knob used for?
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To adjust the distance between the eyepieces
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The Manual WBC Count is used for what purpose(s)?
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1)Monitoring infections
2)Radiation and chemotherapy 3)Following the course of a disease process 4)Indication of leukocytosis/leukopenia |
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How is the counting chamber loaded?
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By touching the tip of the pipette against the edge of the cover slip and the surfaces of the counting chamber (1-1.5 drops)
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What is the formula for determining WBC?
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(Total #/2) x 110% = #.#mm3 |
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What is the distance between the cover slip and hemocytometer platform?
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0.1mm |
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What is the purpose of placing a piece of dampened tissue in the petri dish?
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To keep the hemacytometer from drying out |
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How do you know you have a properly loaded counting chamber?
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A thin, even film of fluid under the cover slip.
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Which cells in a square should be counted?
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The cells touching the top and on the left
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If the difference between the two chambers is greater than what number, does the test need to be repeated?
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20 |
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How long should be given for the cells to settle?
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10 minutes |
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What is the etiology of Leukopenia?
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1)Chronic disease/Idiopathic
2)Overwhelming infections 3)Anaphylactic Shock 4)Radiation/chemotherapy 5)Aplastic anemia 6)Drug induced 7)Bone marrow disease |
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Define Leukocytosis
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Abnormally increased WBC count
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What does the acid solution in the Unopette system, do to red blood cells?
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Hemolyzes the red cells
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What structures of the microscope are found in the illumination system?
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1) Light Source
2) Condenser 3) Iris Diaphragm |
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What are some common causes of Leukocytosis?
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1)Systemic or local infection
2)Highly variable 3)Not dianostic |
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Define "Parafocal" of a microscope
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Focusing plane of one objective is the same or very close to other objectives
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What are some examples of diseases associated with Leukocytosis?
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1)Pneumonia...20-30,000
2)Meningitis...20-30,000 3)Appendicitis...10-30,000 |
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Define "Resolving Power" of a microscope
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1) The useful limit of magnification
2) Ability of the scope to distinguish two objects close to one another |
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What does the base of a microscope do?
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1) structure on which the microscope rests
2) houses power switch, illumination controls, field diaphragm and other various control switches depending on make and model |
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What microscope structures are found in the framework system?
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1) Base
2) Arm 3) Mechanical Stage |
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Name the four major systems of the microscope
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1)Framework System
2)Illumination System 3)Magnification System 4)Adjustment System |
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What is the purpose of the mechanical stage on a microscope?
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1)mechanical platform that supports the specimen being examined and moves the specimen on an X\Y axis
2)specimen holder, consists of spring-loaded clips or calipers, secures the specimen to the stage |
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What conditions can represent with an increased WBC of 15,000mm3 due to physiological conditions?
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1)Newborns
2)Pregnancy 3)Severe pain 4)Exercise 5)Cold baths 6)Emotional upset |
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What does the arm of a microscope do?
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supports the magnification and adjustment systems
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What is the purpose of the revolving nose piece?
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1) holds the objective lenses in place
2) turns to change objectives |
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Name some causes of increase/decreased WBC counts
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1)Diseases
2)Therapeutic treatments 3)Pregnancy (I) 4)Newborns (I) 5)Severe pain (I) 6)Exercise (I) 7)Cold bath (I) 8)Emotional upset (I) |
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The most common abnormal or pathological condition for Leukocytosis is?
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Leukemia/Cancer |
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What parts of the microscope are found in the magnification system?
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1)Revolving nose piece
2)Objective Lenses 3)Body Tube 4)Ocular Lenses |
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What does the light source of a microscope do?
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A built-in light source usually a halogen bulb housed in the back end of the base
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What is the purpose of the Unopette system?
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A method for standardization and dilution of blood and other body fluids for increased safety, accuracy, and precision
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What is the purpose of the iris diaphragm?
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controls the amount of light reaching the condenser
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What is the job of the condenser of a microscope?
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1) compact lens system between the light source and the stage
2) concentrates the light and provides a cone of light |
