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23 Cards in this Set
- Front
- Back
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Artificial Chromosome |
Large DNA ClonesYAC (yeast)- 1000 Kb, unstable.BAC (bacterial)- 300 Kb, based on F fertility factor |
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BAC |
300 KbF Fertility Factor |
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Biotechnology |
Use organisms to farm form useful products. |
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cDNA Synthesis & Reverse Transcriptase |
Synth Dbl-stran DNA from RNA template (mRNA)->> Used to construct cDNA.Discovered by Temin and Baltimore |
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Cloning |
generating high amounts of genetically identical DNA molecules |
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Cloning vectors and creating recombinant DNA |
4 Types1. Plasmid2. Phages and Viruses3. Cosmids.4. Artificial Chromosomes |
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Construction of Genomic Libraries |
Gene of interest is not sequences on chromosome.Cleave genomes and cloning fragments to vectors.Screened for gene of interests.cDNA may also be construction for DNA fragments w/out intron sequence. |
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Cosmids |
Hybrid Vectors.Carry large DNA clones.Both Phage & plasmid qualities- Marker, cloning site (pasmid) cos site (phage)- Phage introduce recombinant DNA into E. coli |
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Genetic Engineering |
Changing the genetic info of an organism by changing the sequence of nucleic acids in its genome |
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Industrial Microbiology |
Use microbes to make compounds or use microbes as products. |
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Jackson Symons Berg |
First recombinant moleculesforeign DNA can be recombined not plasmid- replicate and maintain foreign fragment vectors: carriers of foreign DNA |
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Plasmids |
Replicate autonomosouly & easily purified.Requires->> OpenReadingFrame->> Selectable markerMulticlone site for poly linker->>> Site to allow gene to be cloned to placed into plasmid vector |
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Recombinant DNA Technology |
Procedures to carry out genetic engieering |
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Restriction Enzyme |
Bind at regulatory sitesSticky ends.Blunt ends. |
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Steps in Cloning |
- Isolate DNA- use PCR to create copies of DNA fragments- Insert DNA fragments to generate recombinant molecules- Introduce into new host |
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Termin & Baltimore |
Synthesized sDNA from RNA template (mRNA) |
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YACS |
1000 KbUnstable |
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Cloning |
generating high amounts of genetically identical DNA molecules |
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Genetic Engineering |
Changing the genetic info of an organism by changing the sequence of nucleic acids in its genome |
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Recombinant DNA Technology |
Procedures to carry out genetic engieering |
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Biotechnology |
Use organisms to farm form useful products. |
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Industrial Microbiology |
Use microbes to make compounds or use microbes as products. |
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Cosmids |
"Hybrid Vectors. |
