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12 Cards in this Set
- Front
- Back
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Made up of what chains?
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2 identical heavy chains
2 identical light chains |
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What are the 4 domains of the heavy chains?
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Head, Neck, Stalk and Tail
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Describe the Head domain.
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Binds to MT
Binds/Hydrolyzes ATP |
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Describe Neck Domain
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Undergoes conformational changes and works along with the head domain
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Describe the stalk domain
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Functions in structural support
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Describe the tail domain
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Binds to light chains. The light chains then bind to cargo and help in determining cargo.
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What is the step size of kinesin?
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8 nm
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What is the velocity of kinesin?
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The velocity is directly proportional to ATP concentration. The greatest velocity possible is 2 µM/sec
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How many heads does Kinesin have and what is their function?
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2 heads, function in providing stability. 1 head is always bout to MT
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Describe the hand-over-hand model for how Kinesin steps along MT.
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The 2 heads carry out identical but alternating movements. First one leads, the other is lagging. The lagging one moves forward and takes the lead. Similar to walking. Requires the lagging head to twist 180º around the leading hand
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Describe the inch worm model for how kinesin steps along MT.
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1 head always leads and the 2nd head shuffles up just behind it. Does not require 180º rotation
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What do most KRP do?
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1. Mitosis/spindle formation: MT dynamics.
2. Important in interphase and intracellular motility. Stabilize MT- (+) ended motor proteins |
