Reading...
Play button
Play button
Use LEFT and RIGHT arrow keys to navigate between flashcards;
Use UP and DOWN arrow keys to flip the card;
H to show hint;
A reads text to speech;
12 Cards in this Set
- Front
- Back
|
---First Cycle PCR---
Step 1: *-* to separate dsDNA Step 2: *** of primer Step 3: *-* to cause *** w/--- |
Heat to separate dsDNA
Hybridization of primers Cooldown - DNA synthesis - "heat resistant DNA Polymerase" |
|
|
Bacterial 'PCR method'
Step 1: *** is cleaved w/*** Step 2: and "*** to be cloned" and *** is added.. |
1) Cloning vector cleaved w/restriction nuclease.
2) DNA --- DNA Ligase |
|
|
Bacterial 'PCR method'
Step 3: *1* introduced into bacterial cell. Step 4: Cells are *** Step 5: plasmid is *** from..... |
3) ds recombinant plasmid
4) Cells are culture 5) purified from lysed bacteria |
|
|
---Creating Genomic DNA Library---
Step 1) Cut *** & *** *** w/restriction enzyme Step 2) *** together |
1) DNA - plasmid vector
2) Ligate |
|
|
How is cDNA made?
Step 1) Lyse cells & purify --- Step 2) *** with --- primer Step 3) Create *** with --- |
1) mRNA
2) Hybridize - poly-T 3) cDNA - Reverse Transcriptase |
|
|
---Creating Genomic DNA/cDNA Library---
Step 3) *** bacteria with plasmid Step 4) Grow bacteria w/*** as a ***. Step 5) *** become part of library |
3) Transfect
4) antibiotic resistance --- selectable marker 5) Surviving colonies |
|
|
How is cDNA made?
Step 4) Degrade *** with *** Step 5) Synthesize ******* w/----------- ... *** acts as the primer |
4) RNA - RNase
5) '2nd cDNA strand' w/"heat resistant DNA Polymerase" RNA fragment |
|
|
---Creating cDNA Library---
Step 1) mRNA into *** Step 2) *** into *** Step 3) *** inserted into *** *** |
1) cDNA
2) cDNA - dsDNA 3) dsDNA - plasmid vector |
|
|
--- MUSCLE CONTRACTION ---
1) VD *** influx into *** ***. 2) Vesicles empty *** into *** *** |
1) Ca2+ - presynaptic
2) acetylcholine - synaptic cleft |
|
|
--- MUSCLE CONTRACTION ---
3) These bind *** *** receptor |
3) nicotinic acetylcholine
|
|
|
--- MUSCLE CONTRACTION ---
4) Lig Gated ***/*** ***/*** 5) *** spreads & continues ***-*** coupling |
4) Na+/K+ influx/efflux - cytosol
5) Depolarization - excitation-contraction coupling |
|
|
*** is made with poly-t primer
|
cDNA
|
