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30 Cards in this Set
- Front
- Back
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Restriction endonuclease cuts are either *** or ***.
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blunt or staggered
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***: Enzyme that cuts DNA at specific recognition nucleotide sequence.
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Restriction endonuclease
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*** is used to describe the binding of a DNA probe/primer to a DNA strand.
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Annealing
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*** - The process where complementary DNA/RNA form a doublestrand.
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Hybridize/hybridization
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Third PCR cycle creates ***.
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8 dsDNA
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A *** is separate and replicates independently of chromosomal DNA.
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Plasmid
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Chain termination DNA Sequencing
Q3) What's in ALL reaction tubes? 1) --- & --- 2) four----------(---) 3) --- & --- |
1) Buffer & Primer
2) four dNTPs(deoxynucleotides) 3) DNA polymerase & cofactor magnesium |
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YAC?? composed of...
1) 2) 3) "___ ___ ___" |
Yeast artificial chromosome
1) Left Arm 2) Right Arm 3) Large chromosomal fragments. |
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There are *** reading frames for dsDNA.
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6
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*** are shown as higher peaks during DNA sequencing.
They are... |
Microsatellites
1-6 DNA base pairs |
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Introns in a genomic DNA library are...
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present and interspersed
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"Separate the DNA strands and *** ***"
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anneal primers
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Chain termination DNA Sequencing
Q3) What's unique in each reaction tube? 1) --- |
ddNTP
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dNTP?
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deoxynucleotide triphosphate
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ddNTP?
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dideoxynucleotide triphosphate
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Teacher stressed, PCR uses ***.
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"heat resistant DNA Polymerase"
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Methods of DNA amplification...
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PCR
Bacterial amplification |
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Methods to analyze DNA...
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VNTR
mircoarray |
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PCR?
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Polymerase Chain Reaction
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A *** is a map of known restriction sites within a sequence of DNA.
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restriction map
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Bacteria used in Genomic DNA Library have been *** with a ***.
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transfected
plasmid vector |
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Average cell membrane contains *** phospholipids.
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1 trillion
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Introns in a cDNA library are...
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absent
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genomic DNA clones used to create ***
cDNA clones used to create *** |
genomic DNA library
cDNA library |
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cDNA?
cDNA library represents ***... |
complementary DNA
all mRNA transcribed |
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Glucose concentration...
Intestinal lumen? epithelium? Extracellular? |
low
high low |
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VNTR?
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Variable Number Tandem Repeat
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VNTR from *** *** of mother/father used with *** & *** for paternity/maternity testing.
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homologous loci
PCR & electrophoresis |
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When analyzing VNTRs, *** are used for *** amplification.
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primers
PCR |
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VNTR analysis at four loci will have *** bands. VNTR w/largest # of repeats will be at the ***.
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8 or less
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