Jz Uhcl Cellular Physiology Exam 2 Important

Front 1

Chain termination DNA Sequencing---
Q1) DNA sequence is read from ----.(***)
Q2) Bottom bands are the *** fragments.

Back 1

Q1) bottom of the gel upward(AFTER primer)
Q2) shortest

Front 2

*** EXPLAIN ***
Chain termination DNA Sequencing
1) dsDNA > ???
2) Add ??? to reaction tubes ..............

Back 2

1) ssDNA
2) ssDNA - A, T, G, C

Front 3

*** EXPLAIN ***
Chain termination DNA Sequencing
3) heat to *** & *** according to size by ---.
4) Analyze with ***/*** film OR ***/gel ***.

Back 3

3) denature & separate - gel electrophoresis
4) autoradiography/x-ray film
UV light/gel image

Front 4

FIVE CELL TERMS
3/5 - Outside To Inside

Back 4

Apical plasma membrane
Lateral plasma membrane
Basal plasma membrane

Front 5

FIVE CELL TERMS
2/5 - Outside To Inside

Back 5

tight junction
basal lamina

Front 6

Epithelial cells have two surface compositions called *** & ***.

Back 6

basolateral membrane
apical membrane

Front 7

FRAP?

Back 7

Fluorescence recovery after photobleaching

Front 8

How does FRAP work?
1) small *** is ---
2) t---...
3) b--- d---

Back 8

1) Small area is bleached
2) Time...
3) Bleached dissipates

Front 9

DRAW FRAP diagram

Back 9

Front 10

Tight junctions AKA ***.

Back 10

zonula occluden

Front 11

Name the four ways lipids move around?
1) Common
2)
3)
4) Rare

Back 11

Lateral diffusion
Flexion - break a pencil
Rotation - spinning
Flip-flop

Front 12

Hybridoma experiment.
1) - & - cell
2) form ----

Back 12

1) Mouse & human cell
2) Form heterocaryon

Front 13

Hybridoma experiment.
3) components

Back 13

3) mouse Ig w/ fluorescein
human Ig w/ rhodamine

Front 14

Hybridoma experiment.
4) results

Back 14

0 minutes = 50/50 split
40 minutes = complete diffusion

Front 15

--- Ion Concentration gradients INSIDE cell ---
Higher?
Lower?

Back 15

Higher - K+
Lower - Na+, Ca++, Cl-

Front 16

Glucose uptake in intestinal epithelial cells is driven by this common mechanism...
--- --- --- of *** ***.

Back 16

Potential energy gradient of second molecule

Front 17

First glucose uptake protein? ***
Second? ***
This helps too... ***

Back 17

Na+ driven glucose symport - Na+/Glucose IN
passive glucose transporter - Glucose out
Na+/K+ pump - Na+ out and K+ in

Front 18

ABC-transporter?

Back 18

ATP-binding cassette transporter

Front 19

***(***) is the prototype and most extensively-studied ***.

Back 19

Pgp (P-glycoprotein)
ABC transporter

Front 20

Pgp is a problem in *** because it transports drugs like...
***

Back 20

chemotherapeutic treatment
adriamycin

Front 21

MDR?

Back 21

Multidrug resistance

Front 22

1) What is the problem with MultiDrug Transporter?
--- *** of *** *** w/*** ***
2) Why does it occur?
--- *** *** a*** h*** s***

Back 22

1) efflux of xenobiotic compounds w/broad specificity
2) defense mechanism against harmful substances.

Front 23

***: chemical present but not normally produced by the body.
Greek?

Back 23

Xenobiotic
xenos = foreigner
bios = life
-ic = adjective

Front 24

***: word/phrase naming an attribute.

Back 24

adjective

Front 25

Draw FRAP process....

Back 25

Front 26

***: a collection of microscopic DNA spots attached to a solid surface.

Back 26

DNA microarray

Front 27

DNA microarray is used to measure...

Back 27

gene expression

Front 28

DNA microarray steps...
1) --- & --- tagging
2) --- & wash
3) analyze w/---

Back 28

1) red & green fluorescent tagging
2) hybridize & wash
3) fluorescence microscope

Front 29

Microarrays use strands of *** to ***.

Back 29

DNA to complement mRNA

Front 30

1) Three parts of cholesterol.

4) How long?

Back 30

1) polar head group
2) rigid steroid ring structure
3) nonpolar hydrocarbon tail
4) 2nm

Front 31

*** is a drug that utilizes lysosomes.

Back 31

Doxorubicin

Front 32

---CELL MEMBRANE PERMEABILITY---
?3? - hydrophobic molecules

Back 32

O2 - N2 - steroid horomones
highly

Front 33

---CELL MEMBRANE PERMEABILITY---
?3? - small uncharged polar molecules

Back 33

h2o - urea - glycerol
occasionally

Front 34

---CELL MEMBRANE PERMEABILITY---
?2? - large uncharged polar molecules

Back 34

glucose - sucrose
rare

Front 35

---CELL MEMBRANE PERMEABILITY---
?5? - ions molecules

Back 35

H+ - Na+ - K+ - Cl- - HCO3-
never

Front 36

***: Glial cells of the CNS.

Back 36

Oligodendrocytes

Front 37

Neurotransmitters bind to *** on *** and cause an inrush of Ca++.

Back 37

transmitter-gated ion channels
dendrites

Front 38

*** is a good endonuclease restriction enzyme.

Back 38

HpaI
I=roman numberal
Haemophilus parainfluenzae

Front 39

Lipid bilayer is *** thick.

Back 39

5nm

Front 40

1) PE?
2) PS?

Back 40

Phosphatidyl-
1) ethanolamine
2) serine

Front 41

NANA is also known as ***. It is known for...

Back 41

Sialic acid
net negative charge in ECM

Front 42

*** charge in cytosol... *** on outside.

Back 42

-
+

Front 43

The *** is what is duplicated in bacteria during molecular cloning.

Back 43

recombinant plasmid

Front 44

BAC?

Back 44

Bacterial artificial chromosome

Front 45

Only the creation of a *** library uses restriction nuclease ***.

Back 45

genomic DNA
digestion

Front 46

Bacteria used in Genomic DNA Libraries have a *** for identification.

Back 46

selectable marker

Front 47

Membrane protein that uses...
mutiple alpha helixes

Back 47

multipass transmembrane protein

Front 48

Membrane protein that uses...
covalent bonds on outer monolayer

Back 48

partial anchoring protein

Front 49

(S)*** are found in the cytosol.

Back 49

sulfhydryl groups

Front 50

Transmembrane proteins can be limited to regions through a --- and ---.

Back 50

membrane 'skeleton' and associated proteins

Front 51

Channels control by these means...

Back 51

1) voltage-gated
2) ligand-gated(extra/extracellular ligand)
3) mechanically gated

Front 52

States of Na+ channels...

Back 52

Closed
Inactivated
Open

Front 53

***: action potential of myelinated axons....
***: action potential of nonmyelinated axons....

Back 53

Saltatory conduction
action potential conduction