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46 Cards in this Set
- Front
- Back
glycosidic bond reactions
2 mono >>> di di >>> 2 mono |
condensation
hydrolysis |
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Numbering rule for sugar carbons
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USE PRIME
' |
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Draw glycerol
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make note of H on glycerol...
h2 - h - h2 |
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Element in amide group...
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N
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Phospholipid components
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head group
phosphate glycerol (2) fatty acid |
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AA
1) left side? 2) right side? |
N - amino group
C - carboxyl group |
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kinky AA?
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proline
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disulfide bond AA?
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cysteine
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RNA different from DNA
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sugar: ribose NOT deoxyribose
Uracil NOT Thymine SS not DS |
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Nucleotides?
U - T - A - G - C |
Uracil
Thymine Adenine Guanine Cytosine |
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Purine mnemonic?
Purines? |
PURe As Gold
Adenine Guanine |
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Pyrimidine mnemonic?
Pyrimidines? |
--- CUT PYe ---
Cytosine Uracil Thymine |
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More electrons...
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reduced
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Less electronics...
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oxidized
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High energy ATP bond
?-?-? |
phosphoanhydride bond
O-P-O |
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The donor atom usually has the ??? atom?
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H
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mole?
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approx. 6.022×10^23
# of C12 isotope atoms in 12 grams |
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molarity
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measure of concentration
solute(mol) / solution(L) |
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Draw two generic AA structures...
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H2N-C-COOH ------ H3N(+)-C-COO(-)
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AA Backbone string atoms...
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CCN...
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How many bonds does AT have?
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2
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How many bonds does GC have?
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3
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GFP?
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green fluorescent protein
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Name two types of sedimentation centrifuges...
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Velocity sedimentation
Equilibrium sedimentation |
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Chromatography does what ....
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separates stuff
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Proteins almost always have a *** charge.
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negative
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Ion-Exchange Chromatography separates by ***. It contains *** *** beads.... Final step is...
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charge -
positively charged - (Elution)flush w/solution |
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Gel-Filtration Chromatography separates by ***. *** go through faster... then ***
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size -
bigger particles - Elution |
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Affinity Chromatography separates by ***... then ***
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use of antibodies
elution |
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mAB?
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monoclonal anibody
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These cells secrete antibodies...
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b-cells
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Antibody tail is ***. Top binding side is*** and bottom is ***.
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heavy-chain -
heavy-chain - light-chain |
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*** and *** use Ig to identify targets for phagocytosis.
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Macrophages -
Neutrophil |
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1) Primary antibody?
2) Secondary antibody? |
1) binds to antigen
2) binds to antibody |
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What? Why?
Take out *** organ. |
spleen
home of Ig |
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Ig class switching... constant region of *** is changed but variable region stays the same.
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heavy chain
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Draw triacylglycerol... another name for it?
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make note of H on glycerol
Triglyceride |
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DNA ribose 5' carbon has *** group.
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phosphate
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DNA ribose 3' carbon has *** group.
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hydroxyl
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ΔG+ reaction will happen?
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NO
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ΔG- reaction will happen?
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YES
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TEM?
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Transmission Electron Microscope
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SEM?
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Scanning Electron Microscope
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Name parts of centrifuge
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motor
rotor armored chamber |
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Name two things done to centrifuge chamber?
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refrigeration
vacuum |
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Liquid at top of centrifuged vial?
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supernatant
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