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46 Cards in this Set

  • Front
  • Back
glycosidic bond reactions
2 mono >>> di
di >>> 2 mono
condensation
hydrolysis
Numbering rule for sugar carbons
USE PRIME
'
Draw glycerol
make note of H on glycerol...
h2 - h - h2
Element in amide group...
N
Phospholipid components
head group
phosphate
glycerol
(2) fatty acid
AA
1) left side?
2) right side?
N - amino group
C - carboxyl group
kinky AA?
proline
disulfide bond AA?
cysteine
RNA different from DNA
sugar: ribose NOT deoxyribose
Uracil NOT Thymine
SS not DS
Nucleotides?
U - T - A - G - C
Uracil
Thymine
Adenine
Guanine
Cytosine
Purine mnemonic?
Purines?
PURe As Gold
Adenine
Guanine
Pyrimidine mnemonic?
Pyrimidines?
--- CUT PYe ---
Cytosine
Uracil
Thymine
More electrons...
reduced
Less electronics...
oxidized
High energy ATP bond
?-?-?
phosphoanhydride bond
O-P-O
The donor atom usually has the ??? atom?
H
mole?
approx. 6.022×10^23
# of C12 isotope atoms in 12 grams
molarity
measure of concentration
solute(mol) / solution(L)
Draw two generic AA structures...
H2N-C-COOH ------ H3N(+)-C-COO(-)
AA Backbone string atoms...
CCN...
How many bonds does AT have?
2
How many bonds does GC have?
3
GFP?
green fluorescent protein
Name two types of sedimentation centrifuges...
Velocity sedimentation
Equilibrium sedimentation
Chromatography does what ....
separates stuff
Proteins almost always have a *** charge.
negative
Ion-Exchange Chromatography separates by ***. It contains *** *** beads.... Final step is...
charge -
positively charged -
(Elution)flush w/solution
Gel-Filtration Chromatography separates by ***. *** go through faster... then ***
size -
bigger particles -
Elution
Affinity Chromatography separates by ***... then ***
use of antibodies
elution
mAB?
monoclonal anibody
These cells secrete antibodies...
b-cells
Antibody tail is ***. Top binding side is*** and bottom is ***.
heavy-chain -
heavy-chain -
light-chain
*** and *** use Ig to identify targets for phagocytosis.
Macrophages -
Neutrophil
1) Primary antibody?
2) Secondary antibody?
1) binds to antigen
2) binds to antibody
What? Why?
Take out *** organ.
spleen
home of Ig
Ig class switching... constant region of *** is changed but variable region stays the same.
heavy chain
Draw triacylglycerol... another name for it?
make note of H on glycerol

Triglyceride
DNA ribose 5' carbon has *** group.
phosphate
DNA ribose 3' carbon has *** group.
hydroxyl
ΔG+ reaction will happen?
NO
ΔG- reaction will happen?
YES
TEM?
Transmission Electron Microscope
SEM?
Scanning Electron Microscope
Name parts of centrifuge
motor
rotor
armored chamber
Name two things done to centrifuge chamber?
refrigeration
vacuum
Liquid at top of centrifuged vial?
supernatant