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41 Cards in this Set
- Front
- Back
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1M solution = ***/***
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1 mole/1L
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1M of H2SO4 = 2M H+
Equivalence factor = *** 1M H2SO4 solution = *** normal |
equivalent factor...
0.5 2N |
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1 molal solution = ***/***
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1mol / 1000g of solvent
1mol / 1kg of solvent |
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1mL of 50X + 24mL diluent
This is a *** dilution with the Dilution Factor being *** |
1/25
25 |
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What is a Bronsted acid?
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proton donor
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What is a Bronsted base?
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proton acceptor
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What is autoionization?
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spontaneous formation of ions
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EQUATION for dissociation constant?
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Keq=
[H+][OH-] ---------- [H2O] |
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pH = pKa
[HA](***) concentration is *** [A-](***) concentration. |
acid - equal - base
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pH > pKa
[HA](***) concentration is *** [A-](***) concentration. |
acid - less - base
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pH < pKa
[HA](***) concentration is *** [A-](***) concentration. |
acid - greater - base
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Buffers must be +/- *** pH from desired pH......
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1 or 0.5
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To make a buffer you need *** and ***.
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weak acid(or weak base)
and its salt |
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If x = b^y...
*** is the logarithm of *** to base *** Written... y=log***(***) |
y - x - b
logb(x) |
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1000 = 10^3
log***(***) = *** |
log10(1000) = 3
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What equation is used to find the pH of buffer solutions?
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Henderson–Hasselbalch equation
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Chromatography is a method for separating the components of a mixture by *** *** between a stationary phase and a mobile phase.
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differential adsorption
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All chromatographic systems have these common features...
*** phase *** phase *** mixture |
mobile
stationary component |
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Partition coefficient: constant for a given *** with *** but varies w/***.
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pair of solvents
a particular solute temp |
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***: The ratio of concentrations of a compound in the two phases of a mixture of two *** solvents at equilibrium.
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Partition coefficient
immiscible |
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Chromatography: Cellulose w/acetone...
1) a neutral analyte travels w/*** 2) a polar analyte travels w/*** |
1) acetone - fast
2) thin layer of water on cellulose - slow |
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Small molecules in gel filteration chromatography....
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are retarded
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***: Process by which the energy of a photon of electromagnetic radiation is taken up by another entity.
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Absorption
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A *** is used to measure absorbance.
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spectrophotometer
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Reagents *** or *** *** with a chemical of interest that doesn't absorb UV/visible wavelength.
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react
form complexes |
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Only use standard curves when absorbance values fall into the *** region.
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linear
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Sedimentation coefficient?
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the ratio of a particle's sedimentation velocity to the acceleration that is applied to it (causing the sedimentation).
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The sedimentation coefficient has the dimensions of a unit of time and is expressed in ***.
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svedbergs
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Bigger particles tend to sediment faster and thus have higher *** values.
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svedberg
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Two common types of centrifuge rotors...
1) 2) |
1) fixed angle
2) swing out |
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*** law describes the rate of sedimentation of a sphere in a liquid and provides insight into purifying subcellular particles using centrifugation.
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Stoke's
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ρ(***) is commonly used as a unit for ***.
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Rho - density
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Rate zonal centrifugation is...
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separation by size
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Isopycnic centrifugation is...
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separation by density
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Enyzmes are measured in
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M/L
g g/L katal |
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The *** is the SI unit of catalytic activity.
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katal(kat)
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A *** *** can be used to estimate the initial rate of an enzyme.
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progress curve
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Plot of substrate (S) or product (P) against time is called a *** ***.
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progress curve
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Measure enzyme @ start & at a point on the linear phase to estimate ***.
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initial rate/velocity
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The ____ rate of the reaction increase as more substrate is added.
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initial rate/velocity
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Enzymes helps substrate reach its *** and does NOT alter the ***.
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equilibrium
thermodynamics |
