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14 Cards in this Set
- Front
- Back
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DNA bp Standard Curve
1) X-axis variable? 2) Y-axis variable? |
1) log bp
2) migration distance |
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DNA bp Standard Curve
1) stain? |
1) Ethidium bromide
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Reversible inhibition categories?
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1. competitive
2. noncomp 3. uncompetitive |
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--- Competitive Inhibition ---
How does it work? Effects on Km and Vmax? |
Competes for binding site looks like substrate
Km Increases Vmax remains same |
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--- Noncompetitive Inhibition ---
How does it work? Effects on Km and Vmax? |
Binds at a site other than active site
Km remains same Vmax decreased |
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--- Uncompetitive Inhibition ---
How does it work? Effects on Km and Vmax? |
Binds to ES. No product is formed.
Km decreases Vmax decreases |
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Which class of inhibitors:
1) Decreases Vmax & 2) Has no effect on Km |
Noncompetitive
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Competitive?
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Mixed?
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Noncompetitive?
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Pure non-competitive inhibition equation....
Vmax(inhib) = ? |
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Pure non-competitive inhibition equation....
Ki = ? |
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Uncompetitive?
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DNA Plasmid Isolation and Purification w/Silica Beads
How many steps? |
16
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