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12 Cards in this Set
- Front
- Back
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Cooperativity can be defined in terms of the ***.
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Cooperativity Index (Rx)
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---M: monomeric subunit (termed protomer)
---M2 is the dimeric protein. 1) If 1st ligand causes a LARGE affinity increase for the 2nd it has *** Cooperativity. 2) There will be either NO ligand OR saturated w/ligand. |
1) Complete
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A *** is the structural unit of an oligomeric protein.
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protomer
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--- MWC Model ---
•In absence of ligand, equilibrium favors *** state. •Ligand binding shifts equilibrium toward *** state. |
T
R |
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The Adair equation describe binding under the *** model.
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KNF
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1) The classical ligand/protein binding models used the proteins *** and ***.
2) One of these proteins has a ligand that w/*** effects rather than ***. |
1) myoglobin --- hemoglobin
2) homotropic --- heterotropic |
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Myoglobin --- Hemoglobin
1) Binding Sites? 2) Location? 3) Curve? |
1) 1 --- 4
2) muscle --- blood 3) hyperbolic --- sigmoidal |
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1) General binding curve of Langumir equation is...
2) *** is equivalent to the ligand concentration giving ½ fractional saturation. |
1) rectangular hyperbola
2) Kd |
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Hemoglobin transitions between low and high affinity states due to *** w/in it. This results in a *** binding curve
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1) cooperativity
2) sigmoidal |
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--- pO2 ---
1) Lungs? 2) Peripheral tissues? |
1) 13.3 kPa
2) 2 kPa |
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--- Adair Equation ---
For the binding of a ligand to a protein w/2 binding sites for the ligand. 1) M2: two protomers are... 2) M2S 3) M2S2 |
1) both unbound
2) 1 bound protomer/1 unbound 3) both bound |
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--- Adair Equation ---
--Dimeric and NO Cooperativity-- 1) M + S ⇌ MS is the *** binding constant labeled K***. 2) M2 + S ⇌ M2S is the *** binding constant labeled K***. 3) M2S + S ⇌ M2S2 is the *** binding constant labeled K***. 4) Kb1 = ***Kb 5) Kb2 = ***Kb |
1) intrinsic --- Kb
2) apparent --- Kb1 3) apparent --- Kb2 4) Kb1 = 2Kb 5) Kb2 = ½Kb |
