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14 Cards in this Set
- Front
- Back
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Parasitology specimen requirements
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Stool= most common specimen
Fresh with 1 hour of collection: You must collect entire specimen, collect in clean, dry container, free of urine or water |
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Reason for specimen rejection for parasitology testing
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Age of specimen, urine/water contamination, antidiarrheal drugs, antimalarial drugs, antibiotics, bismuth or magnesium, barium enema, more than one specimen collected on the same day
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What are you looking for in the visual exam of specimen during parasitology testing?
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Color
consistency: liquid, loose, soft, hard Gross exam: Worms/proglottids blood, mucous/pus |
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Preservation of stool during parasitology testing
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PVA (polyvinyl alcohol)
10% formalin SAF MIF Schaduin's fixative 1:3 ratio of stool to fixative |
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Other specimens for detecting parasitic infections:
1: urogentital 2: tissue biopsy 3: duodenal aspirate 4: sputum 5: blood film 6: tape prep 7: direct antigen |
1: trichomonas vaginalis
2: pneumocystis carinii 3: giardia lamblia/strongyloides 4: paragonimus ova 5: malaria,babesia 6: pinworm 7: giardia/crypto |
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Concentration method of the Ova and Parasite exam
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Increase number of organisms and reduced fecal debris. This test is based on the specific gravity of parasite vs the debris
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What are the 2 methods used in the concentration method of Ova and Parasite exam
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Sedimentation and floatation
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What is the concentration prep used for the Ova and Parasite exam
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Examines drops of sediment for organisms useing:
Saline: 0.85% solution Iodine: Dobell & O'Conners Scan under low power and then use high power to identify use the ocular micrometer to measure size of any org seen |
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What does the Iodine in the concentration prep for Ova and Parasite do?
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It stains the nuclear material
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You also use permanent stained smears for a ova and parasite what are those stains used?
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1: trichrome stain: used to Identify protozoan trop and cysts
2: Iron hematoxylin stain: used to id protozoan troph and cyts 3: Modified Acid Fast stain: used to id Coccidia: Crytosporidium, Cyclospora, and Isospora You read under Oil immersion |
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What are artifacts that can be seen during an Ova and Parasite exam?
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yeast
WBC's Meat fibers Pollen Granules fungal spores vegetable cells human hairs cloth fibers oil droplets charcot leyden crystals |
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Define charcot leyden crystals
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Break down product of Eosinophils
Presence of these are indicative of a parasitic infection |
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What are the Identifing Characterisitcs during parasitology testing?
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Size: Size is critical for Id
Internal structures number of nuclei nuclear arrangement cytoplamic inclustions |
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What are certain safety issues you should be concerned with during parasitology testing?
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Stool is a biohazard and the reagents are toxic and flammable
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