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79 Cards in this Set

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Two classification of fungi

Molds and Yeasts

Preffered pH of fungi

Neutral pH (pH 7)

What is necessary for the growth of fungi

Moisture

Common modes of transmission of fungi

Inhalation of spores


Direct contact with spores


Inoculation by trauma into the skin


Person to person contact


Animal to person contact

Differentiate cell type of Yeast and Molds

Y: unicelluar


M: Multicelluar

M for M ;)

Differentiate yeast and molds appearance in agar

Y: smooth, creamy, bacterial-like colony without aerial hyphae



M: fuzzy/ wooly appearance

Differentiate how Yeasts and molds are being identified

Y: biochemical testing


M: microscopic appearance

On what temperature does yeast grows

35'C - 37'C

Differentiate yeast and mold reproduction

Y: budding/ fission



M: mostly sexual

M for malibog hahahaha

Involves maturation of the bud to an independent bladtoconidium (daughter cell)

Budding

In this reproduction two cells of equal size are formed

Fission

On what temperature does mold grows

25'C

Part of molds that appears fuzzy and wooly. It is also the intertwining structure composed of tubular filaments called hyphae

Mycelia

Microscopic filaments of fungi

Hyphae

Contains fruiting bodies that produce reproductive structures

Aerial mycelia

Extend downward into the medium to absorb nutrient

Vegetative mycelia or Thallus

Classification of hyphae according to shape

Antler


Racquet


Spiral


Rhizoids

Swollen, branching tips that resemble moose antlers

Antler hyphae

Tightly coiled hyphae

Spiral hyphae

Enlarged, club-shaped areas

Racquet hyphae

Rootlike structures hyphae

Rhizoids

Cross-walls occuring perpendicularly to the outer walls of the hyphae

Septate

Few Cross-walls at irregular intervals

Sparsely septate

Absence of septation, continuous without cross walls

Aseptate

Aseptate has been used to describe the hyphae of?

Zygomycetes

Other term for hyaline

Moniliaceous

Other term for phaeiodb

Dematiaceous

Pigmentation of hyaline

Nonpimegmented / Lightly pigmented

Pigmentation of Phaeiod

Dark pigmented

Where does melanin is present? Hyaline or Phaeiod

Phaeoid

All fungal elements appear black in this stain

Gomori methylene stain

Stains melanin to determine hyphal pigmentation in tissue

Fontana-Masson stain

Differentiate hyphae colors of Phaeiod and hyaleine hyphae in a Fontana-Masson stain

Phaeiod hyphae: brown


Hyaline hyphae: pink to red

It is a fungi that has an ability to exist in two forms dependent on growth conditions

Dimorphic fungi

Two phases of dimorphic fungi and differentiate it

Mold phase: grows at room temp (22'C to 25'C)



Yeast or spherule phase: seen in vivo or grows at 37'C with increased CO2

Thermally dimorphic fungal spp associated with human disease

Blastomyces dermatitidis


Coccidioides immitis


Histoplasma capsulatum var capsulatum


Paracoccidioides brasiliensis


Sporothrix schenckii


Penicillium marneffei

It has has both Yeast and mold forms in the same culture

Polymorphic fungi

What spp are polymorphic in form

Exophilia spp

Formed as a result of asexual reproduction following mitosis

Conidia

Specialized fruiting structures that carries asexual reproduction

Conidiogenous cells

Parent cell enlarges, septum forms and the enlarged portion splits off to form daughter cells

Blastic

Septum forms first and new growth beyond the septum becomes daughter cell

Thallic

Asexual spores that form on the hyphae or conidiophore

Conidia

Specialized fruiting structures

Conidiogenous cells

Vaselike structures thst produce phialoconidia

Conidia

Ringed structures that produce annelloconidia

Annellides

Enumerate conidia formation

Macroconidia


Microconidia


Blastoconidia (blastospores)


Chlamydoconidia


Arthroconidia


Sporangiospores

Spores develop from what form of mycelium

Vegetative mycelium

Daughter cell that buds from mother cell, hyphae or pseudohyphae

Blastoconidia

Formed from rounding up and enlargment of hyphal segments

Chlamydoconidia (chlamydospores)

Fragmentation of the hyphae into barrel or rectangular shape spores

Arthroconidia

Arthroconidia

Contains in sac. Produced at tip of sporangiophore

Sporangiospores (sporangium)

Requires the joining of two compatible nuclei, followed by meiosis. Spore products

Sexual production

Fusion of 2 identical cells from same hyphae. Conjugation fungi

Zygospore

Fusion from 2 separate non identified hyphae

Oospore

Contained in saclike ascus

Ascospore (Sac fungi)

Contained in a club-shaped basidium

Badiospore

These are septate hyphaes

Ascoscpore


Basidiospore

Sexual form

Teleomorph

Asexual form

Anamorph

More than one anamorph is present for the same teleomorph and produces more than one asexual form

Synanamorph

Psedolalleschena boydii is a teleomoprh and has two anamorphs. What are these two anamorphs

Scedosporium boydii and Graphium spp

Most causative agents of clinical fungi infections

Glomeromycota


Ascomycots


Basidiomycota


Fungi imperfecti (deuteromycota)

On what order does phlum glomeromycota belongs

Mucorales

4 genera of mucolares

Lictheimia


Mucor


Rhizomncor


Rhizopus

Describe mucorales

All are soil dwelling


Rapid growers


Opportunistic pathogens


Profuse, gray to white, aerial mycelium


Hyaline: sparsely septate hyphae

Asexual reproduction is characterized by presence of this

Sporangiospores

Asexual spores produced in this structure

Sporangium

Sporangiun develops from a supporting structure called

Sporangiophore

Some of sporangium reproduce sexually to produce?

Zygospore

Characterized by production of sexual spores known as ascospores

Ascomycota

Where does ascospores form

Ascus

Example of a Basidiomycota

Filobasidella neoformans

Perfect sexual form of cryptococcus neoformans

Filobasidiella neoformans

Occur at the septations in the vegetative hyphae

Clamp connections

A portion of the hypha on one side of the septation grows out anf connects to the hypha on the other side of the septum

Clamp connections

Contains the largest no. Of organisms that are causative agents of mycoses

Fungi imperfecti

Filobasidiella neoformans is a perfect sexual form of?

Cryptococcus neoformans

Identified on the basis of characteristic asexual reproductive structures

Fungi imperfecti