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16 Cards in this Set
- Front
- Back
- 3rd side (hint)
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Histology |
- study of tissues - visual science that relies on microscopy to reveal cell, tissue, and organ substructure - visual discipline that combines observation with reasoning |
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1. Fixation 2. Dehydration 3. Clearing 4. Infiltration 5. Embedding 6. Sectioning 7. Mounting 8. Staining |
Enumerate the steps on how to prepare tissues for Microscopy. |
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Fixation |
-preserves structural organization of cells, tissues, and organs - prevents bacterial and enzymatic digestion - insolubilizes components to prevent diffusion - reduces damage in later steps in tissue processing |
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1. Chemical Fixation 2. Physical Fixation |
What are the two types of fixation? |
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Chemical Fixation |
What time of fixation is a common approach wherein the fixatives are used individually or inmixtures?
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Physical Fixation (freezing) |
what type of fixation is used for light or electron microscopy that allows tissue to be sectioned without dehydrationor clearing? |
A faster type of fixation. |
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Formalin |
Give an example of a chemical fixative that can be used individually. |
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Bouin’s fixative |
Give an example of a chemical fixative in a mixture form. |
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rapid penetration |
In the chemical fixation, best results achieved by ___________ of living tissue with fixative. |
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immersion; perfusion |
In chemical fixation, small tissue pieces may be fixed by _________, while entire organs better fixed by ______. |
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Perfusion |
A method where the fixatives are pumped through blood vessels. |
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cryoprotectant |
In physical fixation, the tissue is embedded in _______ (glycerin) |
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DEHYDRATION |
This process prepares fixed tissue for infiltrationwhere it replaces tissue water with organic solvent;commonly, ethanol.
The fixed tissue immersed in series of alcohol–water mixtures with increasing alcohol concentration, to 100% alcohol. |
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CLEARING |
This process prepares fixed tissue for infiltrationwherein the dehydrating agent replaced with clearing agent.
The dehydrated tissue immersed in a series of alcohol-clearing agent mixture. |
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1. Xylene 2. Propylene oxide |
Give two examples of alcohol-clearing agent mixture. |
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INFILTRATION |
This process prepares cleared tissue for embedding. |
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