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32 Cards in this Set
- Front
- Back
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Instrumentation |
Foundation of measurements in modern clinical chemistry setting |
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Basic Principles |
Photometry Luminiscence Electrochemistry Chromatography |
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Photometry |
Light intensity w/o wavelength consideration |
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Photometry examples |
Spectrophotometry Atomic absorption Flame emission Mass spectrophotometry |
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Luminiscence |
Already light with specific wavelength |
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Luminiscence examples |
Flourescence Chemiluminiscence Nephelometry Bioluminiscence |
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Electrochemistry |
Involvement of electricity |
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Electrochemistry examples |
Electrophoresis Potentiometry Amperometry Coulometry |
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Chromatography |
Separation of susbstances based on physical property |
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Chromatography examples |
Gas Liquid Thin layer High performance liquid chromatography(HPLC) |
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Light |
Form of energy that appears to travel in the form of waves has (energy, wavelength, frequency) |
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Energy |
Shorter wavelength; increasing energy |
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Frequency |
Inversely proportional to wave length |
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Planck's formula |
Describes the relationship between wavelength and energy (E=hv law in light assoc. With light) |
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X-ray |
Gamma ray 0.1 |
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E, h, v |
Energy of photon in joules H- constant 6.626x10-34 erg sec V- frequency |
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Violet |
Decreasing wavelength; increasing energy and more frequent |
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Notes |
Color absorbed is different to the color of the solution which is the complementary color |
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Gamma ray |
1.0 nm Shortest on energy; highest |
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UV |
220-380 nm |
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Visible light |
380-750nm |
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Infrared |
750-2000nm |
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Microwave |
4000nm Longest on energy; lowest |
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Electromagnetic radiation |
Photons of energy travrls in waves |
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Frequency and energy |
Inversely proportional to the wavelength (Longest wavelength means low energey and frequency) |
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Wavelength |
Gives light specific color |
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Beer's law |
Used in spectrophotometry States that: concentration of substance is directly proportional to the amount of light absorbed but inversely proportional to the logarithm of transmitted light |
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Absorbance formula |
A=2-log%T |
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%T |
Ratio of radiant energy transmitted diveded by radiance of energy absorbed on the sample.. Where 100%T means NO LIGHT IS ABSORBED Cu=A unknown/ A known x C(constant) |
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Spectrophotometry |
Used in routine chemistry Absorbed light in wavelength(monochromator that isolates light) States that: light absorbed is directly proportional to analyte following beer's law |
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Light source |
Emits visible light |
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Monochromator |
460nm Isolates light |
