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11 Cards in this Set
- Front
- Back
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After running the IPG strip how will the proteins be separated overall?
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by pH
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After running the 2D gel how will the proteins be separated overall?
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pH and size
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What is the purpose of doing the Bradford assay?
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quantify amount of proteins
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What is the purpose of the protein prep step?
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ensure only hemolymph proteins are present
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When should you wear gloves?
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at all times
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What are our experimental groups for this lab?
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cuticles of Tenebrio and Strepsiptera
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Why must we incubate the coverslip in PBS + 10% normal serum (blocking buffer)?
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prevent unwanted binding of antibodies
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From what animal was our second antibody prepared?
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goat anti mouse
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What is the purpose of the second antibody?
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bind 1* antibody/act as flourescent label, 1* antibody would not produce as much flourescence
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What proteins are going to be labeled in this lab?
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Granular cells, integrins, plasmatocytes
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What filters labeled what?
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green = plasmatocytes, red = granular cells, blue = labeled nuclei
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