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17 Cards in this Set
- Front
- Back
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What is a fully human monoclonal antibody?
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-umab, it is a human antibody.
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What is a humanized monoclonal antibody?
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-umab, a murine antibody that is completely replaced with human regions except for hypervariable regions (CDRs, 3 on each arm) that remain murine.
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What is a chimeric monoclonal antibody?
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-ximab, murine antibody with human constant regions (Fc portions).
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What is a murine antiobdy?
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-omab, antibody from a mouse.
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How do you create a monoclonal antibody? (1)
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Transgenic mice; replacement of Ig genes in a mouse with human Ig genes. Immunize the mouse and then produce mAbs.
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How do you create a monoclonal antibody? (2)
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Phage libraries; insertions of human Ig gene into a bacteriophage.
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How do you create a monoclonal antibody? (3)
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Hu-SCID mice. Introduction of human cells into a severely immunocompromised mouse meaning it won't be recognised as foreign and can produce Ig.
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Name two anti-TNF-alpha mAbs.
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1) Infliximab; indicated for RA, Chrohn's and ankylosing spondylitis.
2) Certolizumab; PEGylated Fab fragment of a humanized monoclonal antibody. PEGylation increased its circulatin half life. |
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Name an anti IL-12 & -23 mAb.
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Ustekinumab; abrogates NK, Th1 and Th17 mediated release of pro-inflammatory cytokines.
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Name an anti-BLyS (B cell activating factor).
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Belizumab; BLyS is the anti-dsDNA found in SLE.
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Name an anti-IL-6-R mAb.
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Tocilizumab; appropriate in severe RA.
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Name and anti-CD-20 mAb.
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Rituximab; indicated for RA, CLL and non-Hodgkin lymphoma. Binds CD20 and also activates complement (therefore cross-linking CD-20 +ve cells and targetting them for apoptosis.
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Name an anti-alpha-4-integrin.
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Natalizumab; indicated for Crohn's and MS.
In Crohn's integrin mediated leukocyte diapedesis in inflamed areas of GI tract is the main cause of pathology. Natalizumab stops this. |
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Name a fusion protein. (1)
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Abatacept - CTLA-4 fused to Fc of IgG1.
Competitively antaonises B7 molecules found on DC and interacts with CD28 on T cells therefore downregulating T cell actication. Indicated in RA. |
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Name a fusion protein. (2)
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Etanercept - sTNFR (aka p75 aka CD120b) fused to Fc of IgG.
Indicated in RA, JIA, psoriatic arthritis and ankylosing spondylitis. |
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Name a soluble cytokine receptor antagonist.
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Anakinra; recombinant IL-1RA. Indicated for RA, CAPS and JIA.
Competitive inhibitor of IL-1-alpha/beta for IL-1R |
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Name two recombinant cytokines.
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IFN-beta; indicated for relapsing MS and inhibition of autoreactive T cells.
IL-5; induction of antigen-specific CD4+ CD25+ Tregs. |
