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38 Cards in this Set
- Front
- Back
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can we use agglutination tests to determine titers?
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yes
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detecting Ab to Brucella canis
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-start out with whole bacteria on card
-add serum(test,neg,known pos) -clumping or agglutination=positive -smooth=neg -interpretation:patient has Ab to Brucella canis |
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latex aggultination example
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-coat latex beads
-Abs that react with specific Ag (if using to Dx FPT in a foal then Ab on the bead is antibody against Equine IgG) -Ag (positive looks grainy..neg looks smooth) |
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Latex agg test used to detect Rhematoid Arth Factore in dogs
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most dogs with RA have autoantibodies to their own IgG
-this is one criteria to help with diagnosis |
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Latex agg test used to detect Rhematoid Arth Factore in dogs
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-latex beads coated with Canine IgG
(detect antibodies to dog IgG..normal IgG attached to latex beads...) |
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Coggins test
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double line of precipitation
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RadiAL immunodiffusion tests
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FPT
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Latex agglutination test
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Brucella Abortus
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Hemagglutination tests (HA)
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Indicates presence of specific antibody in the patients serum
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HA
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viruses DO NOT aggultinate the RBC;THEY JUST ATTACH
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Coombs test
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-agglutination tests
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Coombs test
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-diagnosis of IMHA immune mediated hemolytic Anemia
--anti-globulin test -this is a hemiglutination test |
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Hemagglutination INHIBITION test (HI)
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-if antibodies are present we do not see agglutination
-NO hemogglutination=POSITIVE for AB to virus |
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in HI if we get agg then..
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=NEGATIVE
so agglutination=negative=no pellet of RBC |
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HI positive =
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no agglutination (b/c remember laura anoying disease...I'm poss..I already HAVE it..im pos)
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neutralization tests
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est the ability of antibody to neutralize the bio activity of an antigen when mixed with it in vitro (ex BVDV)
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Serum Neut tests
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used to detect Abs in patients serum that neutr viruses &/or bacterial toxins
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SN tests are
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highly specific and very sensitive
-example tthe BVDV SN test (Bovine Viral Diarrhea..) |
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Complement Fixation Test (CFT)
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always activating the classic pathway by ANTIBODY bound to "antigen" results in the generation of membrabe attack complexes (MAC) that can disrupt cell membranes
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CFT
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serum with antibody--->reactive =no lysis
serum with no antibody--->Nonreactive=Lysis |
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(CFT) remember lysis means
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negative (antibody not there to tie up complement)
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Acute vs. convalescent
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acute-serum collected early after onset of illness
conv-serum coll 14-21 days later (convalescent:older) |
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A 4 fold rise (four dilutions) in titre from acute to conv
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is compatible with diag for an active infection
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acute titre
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1:2
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conv titer
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1:64 (diagnostic rise in the titre)
-samples must be tested in parallel |
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TP
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tru positive : a positive test result that correctly id's a positive animla
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TN
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A negative tests result that correctly id's a negative animal
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FP
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A POSITIVE test result that is incorrect..Id's a neg animal as a pos
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FN
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A NEG test result that is incorrect..Id's a pos animal as being neg
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sensitivity %=
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porportion of diseased animals that test pos...ability of test to correctly ID positives
-used for screening animals..DONT WANT positive animals to get thru system..dont really care about the nega yet (or FP) |
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Specificit%=
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insert definition
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Sensitivy ==
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ability is correctly ID POSITIVES
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Specificity=
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ability of test to correctly ID NEGATIVES
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Sensitivity=
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SCREENING
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sorting out false positives=
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specificity
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testing for human HIV we want a...
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Highly sensitive test!
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Western blot is a ....
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highly SPECIFIC test ..this test basically tells us hey we thought you had aides but you really just got a rabies vaccination and you "looked" positive for HIV.
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canine influenza
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screening test
then following with specificity test |
