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15 Cards in this Set
- Front
- Back
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What does immunofluorescence detect ?
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primary Ag Ab reaction
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What is the label in immunofluorescence ?
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fluorescent dye
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What is the fluorescent dye always coupled to ?
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the Ab
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What is the complex read with ?
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fluorescent microscope
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What are the 2 major types of immunofluorescent techniques ?
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Direct
Indirect |
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What does the direct method detect ?
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Detection of Ags on the surface /in tissue sections
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Describe the steps of the direct immunofluorescence.
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Take a glass slide that has the tissue and fixed to it the antigen, then add an Ab-complexed to FITC.
after 30 min at room or 37 deg. wash and study using microscope |
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If fluorescence occur then ...
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We have Ag on the slide
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What do we use the indirect method for ?
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quantitation and determination of Abs in serum
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Describe the steps in indirect immunofluorescence .
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Fix Ag to slide (know concentration) add patients serum with Abs, then Add antiAbs that are labeled with a chromophore , if fluorescence occurs then antigen is present in the serum.
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Indirect immunofluorescence is a ...
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Semiquantitative method and qualitative
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Can a titer be determined in indirect method ?
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yes after doing serum dilutions
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What are the applications of immunofluorescence ?
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detect Abs against syphilis T.pallidum
determine TORCH titers |
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What do TORCH tests include ?
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Toxoplasma TO
Rubella R CMV C HSV herpes simplex H |
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What are TORCH significant in ?
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congenital infections
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