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Immunology tests for fungal infections



How many types of tests there are?

3



List

Tests for fungal Abs and Ags


Skin tests

Immunology tests for fungal infections



3


List

Tests for fungal Abs


Tests for fungal Ags


Skin tests

Immunology tests for Fungal infections



_____________


Tests for fungal Ags


Skin tests

Tests for fungal abs



Results that indicate fungal infections



Negative to positive


3 fold rise in Ab titer


Single high titer

Immunology tests for fungal infections



Tests for fungal Abs


________________


Skin tests

Tests for fungal Ags



Example

Cryptococcal Ag

Immunology tests for fungal infections



Tests for fungal Ags


Example

Cryptococcal Ag



Test?

Latex agglutination

Test for Cryptococcal Ag by latex agglutination



Procedure

Pt. sample + Latex beads



Mix, rotate



Look for agglutination

Test for cryptococcal Ag by latex agglutination



Procedure



________+Latex beads



Mix, rotate



Look for agglutination

Pt. sample



List

CSF


serum

Test for cryptococcal Ag by latex agglutination



Procedure



Pt. sample + __________



Mix, rotate



Look for agglutination

Latex beads



Description

Coated with Anti-Cryptococcus Ab

Test for cryptococcal Ag by latex agglutination



Procedure



Pt. sample + Latex agglutination



Mix and rotate



Look for _______

Agglutination



Interpretation of results

+ Agglutination = + Ag = Presence of Cryptococcal infection

Test for cryptococcal Ag by latex agglutination



+ Agglutination

+ Ag = ?

Presence of Cryptococcal infection

Fungal



Skin test results


List

Positive


Negative


Positive to negative


Negative to positive

Fungal skin tests



How many possible results there are?

4



List

Positive


Negative


Positive to negative


Negative to positive

Fungal skin tests



________


negative


Positive to negative


Negative to positive

Positive



Meaning

Exposure to fungus



Good prognostic sign: development of cell-mediated immunity

Fungal skin tests



Positive


__________


Positive to negative


Negative to positive

Negative



Meaning

No exposure to fungus



Fungal skin test



Positive


Negative


_____________


Negative to positive

Positive to negative



Meaning

Poor prognostic sign: Immunosuppression

Fungal skin test



Positive


Negative


Positive to negative


_______________

Negative to positive



Meaning

Current infection

Serologic testing


Advantages over microbiology testing



How many?

5



List

Serologic testings



- more rapid results


- more sensitive than culture



- Culture is difficult/impossible for some organism



- Only means of detecting past infections



- Monitor the course of disease over time

Serologic testing



5 advantages over microbiology testing

Serologic testings



- faster than


- more sensitive than culture methods



Culture of some organisms are difficult/impossible



Only means of detecting past infection



Can monitor the course of disease over time (Description)

inc Ab titer: active infection


dec Ab titer: recovery

Limitations/Disadvantages of serologic testings

Not definitive b/c?

Possibility of


false positive


false negative

Limitations/Disadvantages of serologic testings



Not definitive b/c

Possibility of false positive or negative results